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2012 , Vol. 0 >Issue 04: 479 - 484

DOI: https://doi.org/10.6023/A1108303

研究论文

肝素化丝素支架作为骨形态发生蛋白-2 缓释载体的研究

  • 马寅孙 ,
  • 蔡玉荣 ,
  • 刘琳 ,
  • 姚菊明
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  • 浙江理工大学材料与纺织学院 先进纺织材料与制备技术教育部重点实验室 杭州 310018

收稿日期: 2011-08-30

  修回日期: 2011-10-10

  网络出版日期: 2011-11-28

基金资助

国家自然科学基金(Nos. 21041003, 51172207)资助项目.

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Heparinized Silk Fibroin Scaffolds as the Sustained Release Carrier of Bone Morphogenetic Protein-2

  • Ma Yinsun ,
  • Cai Yurong ,
  • Liu Lin ,
  • Yao Juming
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  • The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018

Received date: 2011-08-30

  Revised date: 2011-10-10

  Online published: 2011-11-28

Supported by

Project supported by the National Natural Science Foundation of China (Nos. 21041003 and 51172207).

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摘要

骨形态发生蛋白-2(BMP-2)的缓释载体一直是骨组织工程中的研究热点. 本研究通过化学改性制备了两种肝素化丝素支架, 并浸渍吸附BMP-2, 研究了BMP-2 在不同丝素支架样品上的吸附能力、体外释放性能及其对人骨肉瘤细胞MG-63 碱性磷酸酶活性(ALP)的影响. 结果表明, 肝素化丝素支架对BMP-2 具有较强的吸附能力, 并能保持其体外缓慢释放性能; MG-63 细胞在肝素化支架上生长状态良好, 并具有显著的增殖能力, 负载BMP-2 后的肝素化支架能显著促进MG-63 细胞的分化. 因此, 肝素化丝素支架是一种较理想的BMP-2 缓释载体.

关键词： 丝素支架; BMP-2; 碱性磷酸酶; 缓释

本文引用格式

马寅孙 , 蔡玉荣 , 刘琳 , 姚菊明 . 肝素化丝素支架作为骨形态发生蛋白-2 缓释载体的研究[J]. 化学学报, 2012 , 0(04) : 479 -484 . DOI: 10.6023/A1108303

Abstract

Sustained release carrier for the bone morphogenetic protein-2 (BMP-2) is currently a hot subject of research on the bone tissue engineering. In this work, two kinds of heparinized silk fibroin scaffolds were prepared. The binding ability to BMP-2, in vitro sustained release property and the alkaline phosphatase (ALP) activity of the scaffolds were investigated. Results showed that the heparinized silk fibroin scaffolds could bind BMP-2 well and sustained release the BMP-2. The scaffolds with BMP-2 could improve the MG-63 cell growth and cell differentiation with high ALP activity. So the heparinized silk fibroin scaffolds could be an ideal sustained release carrier for BMP-2.

Key words： silk fibroin scaffold; BMP-2; alkaline phosphatase; sustained release

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