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化学学报 >

2012 , Vol. 0 >Issue 05: 649 - 658

DOI: https://doi.org/10.6023/A1105024

研究论文

谱图检索结合二级质谱定性分析烟草中挥发性成分

  • 吴丽君 ,
  • 段佳 ,
  • 李倩倩 ,
  • 曹金莉 ,
  • 刘玮 ,
  • 闵顺耕
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  • a 中国农业大学理学院应用化学系 北京 100193;
    b 云南省烟草公司大理州公司 云南 671000

收稿日期: 2011-05-02

  修回日期: 2011-10-10

  网络出版日期: 2011-11-28

基金资助

云南省烟草公司(No. 09YN016)资助项目.

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Identification of the Volatile Components in Tobacco by Gas Chromatograph Coupled with Ion Trap Tandem Mass Spectrometry

  • Wu Lijun ,
  • Duan Jia ,
  • Li Qianqian ,
  • Cao Jinli ,
  • Liu Wei ,
  • Min Shungeng
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  • a Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193;
    b Tobacco Corporation of Yunnan Dali, Yunnan 671000

Received date: 2011-05-02

  Revised date: 2011-10-10

  Online published: 2011-11-28

Supported by

Project supported by Tobacco Corporation of Yunnan Dali (No. 09YN016).

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摘要

采用同时蒸馏萃取法提取烟叶中的挥发性成分, 利用气相色谱-质谱联用仪(GC-MS)分离测定, 通过谱库检索和匹配度定性结合色谱保留指数方法鉴定烟叶中挥发性成分, 并引入离子阱二级质谱对谱库检索匹配度差距小、含量低,背景干扰大的物质准确定性. 采用谱图检索结合二级质谱定性共鉴定144 个化合物, 其中104 个化合物在烟草挥发性成分的文献中已有报道, 报道中有9 个化合物是通过二级质谱定性, 其余40 个化合物还未见报道. 结果表明, 离子阱二级质谱定性的引入提高了对未知化合物定性的准确性和可靠性, 适合于烟叶这类复杂植物体系的化学组分研究.

关键词： 同时蒸馏萃取; 烟叶; 挥发性成分; 离子阱二级质谱

本文引用格式

吴丽君 , 段佳 , 李倩倩 , 曹金莉 , 刘玮 , 闵顺耕 . 谱图检索结合二级质谱定性分析烟草中挥发性成分[J]. 化学学报, 2012 , 0(05) : 649 -658 . DOI: 10.6023/A1105024

Abstract

The volatile components in tobacco leaves were extracted by simultaneous distillation and extraction (SDE), and analyzed by gas chromatography-mass spectrometry (GC-MS). The volatile components in tobacco leaves were identified by library searching and spectral matching combined with retention index. Ion trap tandem mass spectrometry was introduced to identify some components which had tiny difference of library matching, low concentration, and complex matrix. 144 components were identified by library searching, spectral matching and ion trap tandem mass spectrometry in this paper. And 104 volatile components have been reported, 9 of them were identified by ion trap tandem mass spectrometry, the other 40 components have not been reported in the literature. So ion trap tandem mass spectrometry is the precise and reliable method to identify some unknown components, and is suitable for separation and identification of complex system.

Key words： simultaneous distillation and extraction; tobacco; volatile components; ion trap tandem mass spectrometry

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