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化学学报
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化学学报 >

2012 , Vol. 70 >Issue 06: 714 - 720

DOI: https://doi.org/10.6023/A1107051

研究论文

甲醇-水混合溶剂后处理制备丝素蛋白膜及其药物释放研究

  • 韦俏娜 ,
  • 马林 ,
  • 黄爱民 ,
  • 杨华 ,
  • 龚珠萍 ,
  • 强盼盼 ,
  • 张丽
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  • 广西大学化学化工学院 南宁 530004

收稿日期: 2011-07-05

  修回日期: 2011-11-16

  网络出版日期: 2011-12-09

基金资助

国家自然科学基金(No. 21063002), 广西自然科学基金(No. 2011jjC30004)和广西研究生创新计划(No. 105931003028)资助项目.

收起

Controlled Drug Release from Silk Fibroin Films Treated with Methanol-water Mixtures

  • Wei Qiaona ,
  • Ma Lin ,
  • Huang Aimin ,
  • Yang Hua ,
  • Gong Zhuping ,
  • Qiang Panpan ,
  • Zhang Li
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  • College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004

Received date: 2011-07-05

  Revised date: 2011-11-16

  Online published: 2011-12-09

Supported by

Project supported by the National Natural Science Foundation of China (No. 21063002), Natural Science Foundation of Guangxi Province (No. 2011jjC30004) and Post-graduate Innovation Program of Guangxi Province (No. 105931003028).

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摘要

采用溶液浇注法制备丝素蛋白薄膜, 应用傅里叶红外光谱(FTIR)和X 射线衍射(XRD)研究了浓度不同的甲醇-水混合溶剂处理后丝素蛋白薄膜的结构变化, 并以罗丹明B 为模型药物与丝素蛋白构建药物缓释体系, 考察了丝素蛋白膜的结晶结构对药物释放动力学的影响. 结果显示, 在甲醇体积比浓度ΦMeOH＝50%～90%的范围内, 丝素蛋白材料中以β-折叠为主的silk Ⅱ 结晶含量随着混合溶剂中甲醇浓度的增加而先增加后下降, 在ΦMeOH＝80%附近出现最大值. 罗丹明B 从丝素蛋白膜的释放属于Fickian 扩散机理, 其扩散指数n 随着丝素蛋白膜中β-折叠含量的增加而增加, silk Ⅱ结晶是丝素蛋白材料药物释放的天然调节器.

关键词： 丝素蛋白; 药物缓释; 结构变化

本文引用格式

韦俏娜 , 马林 , 黄爱民 , 杨华 , 龚珠萍 , 强盼盼 , 张丽 . 甲醇-水混合溶剂后处理制备丝素蛋白膜及其药物释放研究[J]. 化学学报, 2012 , 70(06) : 714 -720 . DOI: 10.6023/A1107051

Abstract

Silk fibroin (SF) films were prepared using a casting method and the structural change owing to treatment with methanol-water mixtures of different concentrations was investigated by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The results were used to reveal the influence of structure on the release dynamics of Rhodamine B, a model compound loaded in the SF matrix. In methanol concentration ranging from 50% to 90%, the content of silk Ⅱ crystalline structure, constituted by β-sheet, first increased and then decreased with increasing methanol concentration in the mixtures, reaching a maximum around 80%. It was found that Rhodamine B released from the SF film via Fickian mechanism, of which the diffusional exponent increased with increasing β-sheet content in the matrix, suggesting that the silk Ⅱ crystal could be used as natural regulator for drug release from SF material.

Key words： silk fibroin; controlled release; structural change

参考文献

1 Wenk, E.; Meinel, A. J.; Wildy, S.; Merkle, H. P.; Meinel, L. Biomaterials 2009, 30, 2571.  

2 Uebersax, L.; Mattotti, M.; Papaloïzos, M.; Merkle, H. P.; Gander, B.; Meinel, L. Biomaterials 2007, 28, 4449.  

3 Karageorgiou, V.; Meinel, L.; Hofmann, S.; Malhotra, A.; Volloch, V.; Kaplan, D. L. J. Biomed. Mater. Res. 2004,71A, 528.

4 Uebersax, L.; Merkle, H. P.; Meinel, L. J. Controlled Release2008, 127, 12.  

5 Lovett, M.; Cannizzaro, C.; Daheron, L.; Messmer, B.; Vunjak-Novakovic, G.; Kaplan, D. L. Biomaterials 2007,28, 5271.  

6 Li, M.; Wu, Z.; Lu, S.; Sun, D.; Liao, Z.; Zhang, J. CN1628864A, 2005 [Chem. Abstr. 2006, 146,79446].

7 Fan, H.; Liu, H.; Toh, S. L.; Goh, J. C. H. Biomaterials2008, 29, 1017.  

8 Li, M.; Tao, W.; Lu, S.; Kuga, S. Int. J. Biol. Macromol.2003, 32, 159.  

9 Park, K. E.; Jung, S. Y.; Lee, S. J.; Min, B. M.; Park, W. H. Int. J. Biol. Macromol. 2006, 38, 165.  

10 Li, M.; Wu, Z.; Zhang, C.; Lu, S.; Yan, H.; Huang, D.; Ye, H. J. Appl. Polym. Sci. 2001, 79, 2192.  

11 Kim, U. J.; Park, J.; Kim, H. J.; Wada, M.; Kaplan, D. L. Biomaterials 2005, 26, 2775.  

12 Lu, S.; Wang, X.; Lu, Q.; Zhang, X.; Kluge, J. A.; Uppal, N.; Omenetto, F.; Kaplan, D. L. Biomacromolecules 2010,11, 143.  

13 Lu, S.; Wang, X.; Lu, Q.; Zhang, X.; Kluge, J. A.; Uppal, N.; Omenetto, F.; Kaplan, D. L. Biomacromolecules 2010,11, 143.  

14 Tamada, Y. Biomacromolecules 2005, 6, 3100.  

15 Tsukada, M.; Freddi, G.; Kasai, N.; Monti, P. J. Polym. Sci. B: Polym. Phys. 1998, 36, 2717.  

16 Hais, P. I.; Severcan, F. J. Mol. Catal. B: Enzym. 1999, 7,207.  

17 Byler, D. M.; Susi, H. Biopolymers 1986, 25, 469.  

18 Hae, Y. K.; Park, Y. H. Biomaterils 1999, 73, 2887.

19 Cai, S.; Singh, B. R. Biophys. Chem. 1999, 80, 7.  

20 Xie, M. X.; Liu, Y. Chem. J. Chin. Univ. 2003, 24, 226 (in Chinese). (谢孟峡, 刘媛, 高等学校化学学报, 2003, 24, 226.)

21 Chen, X.; Zhou, L.; Shao, Z. Z.; Knight, D. P.; Vollrath, F. Acta Chim. Sinica 2003, 61, 625 (in Chinese). (陈新, 周丽, 邵正中, Knight, D. P., Vollrath, F. 化学学报,2003, 61, 625.)

22 Chen, X.; Shao, Z.; Knight, D. P.; Vollrath, F. Proteins: Struct., Funct., Bioinf. 2007, 68, 223.  

23 Marsh, R. E.; Corey, R. B.; Pauling, L. Biochem. Biophys. Acta 1955, 16, 61.

24 Konishi, T.; Kurokawa, M. Sen'i Gakkaishi 1968, 24, 550.  

25 Li, M. Z.; Ogisob, M.; Minoura, N. Biomaterials 2003, 24,357.  

26 Kweon, H. Y.; Um, I. C.; Park, Y. H. Polymer 2001, 42,6651.  

27 Gil, E. S.; Frankowski, D. J.; Bowman, M. K.; Gozen, A. O.; Hudson, S. M.; Spontak, R. J. Biomacromolecules 2006,7, 728.  

28 Tamada, Y. Biomacromolecules 2005, 6, 3100.  

29 Kima, U. J.; Park, J. H.; Kim, H. J.; Wada, M.; Kaplan, D. L. Biomaterials 2005, 26, 2775.  

30 Zhang, J. W.; Gu, J. K. Dynamics of Controlled/Sustained Release Dosage Form, Science Press, Beijing, 2009, pp.27～50 (in Chinese). (张继稳, 顾景凯, 缓控释制剂药物动力学, 科学出版社, 北京, 2009, pp. 27～50.)  

31 Horan, R. L.; Antle, K.; Collette, A. L.; Wang, Y. Z.; Huang, J.; Moreau, J. E.; Volloch, V.; Kaplan, D. L.; Altman, G. H. Biomaterials 2005, 26, 3385.  

32 Rigter, P. L.; Peppas, N. A. J. Controlled Release 1987, 5,37.  

33 Colombo, P.; Bettini, R.; Santi, P.; Ascentiis, A.; Peppas, N. A. J. Controlled Release 1996, 39, 231.  

34 Yoshimizu, H.; Asakura, T. J. Appl. Polym. Sci. 1990, 40,1745.  

35 Heslot, H. Biochimie 1998, 80, 19.  
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