研究论文

基于元素指纹的白术产地溯源及其与土壤的相关性研究

  • 王小芝 ,
  • 陈瑶 ,
  • 吴海龙 ,
  • 王童 ,
  • 杨健 ,
  • 付海燕 ,
  • 杨小龙 ,
  • 李旭富 ,
  • 丁玉洁 ,
  • 俞汝勤
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  • a 化学生物传感与计量学国家重点实验室 湖南大学化学化工学院 长沙 410082
    b 湖南工业大学 生命科学与化学学院 株洲 412007
    c 中国中医科学院道地药材国家重点实验室培育基地 国家中药资源中心 北京 100700
    d 中南民族大学 药学院 武汉 430074
    e 北京同仁堂平江白术有限公司 平江 414500

收稿日期: 2021-09-27

  网络出版日期: 2021-12-29

基金资助

国家重点研发计划(2020YFC1712700); 湖南省教育厅科学研究项目(19C0554)

Study on the Origin Traceability of Atractylodes macrocephala Koidz. and Its Correlation with Soil Based on Mineral Elements

  • Xiaozhi Wang ,
  • Yao Chen ,
  • Hailong Wu ,
  • Tong Wang ,
  • Jian Yang ,
  • Haiyan Fu ,
  • Xiaolong Yang ,
  • Xufu Li ,
  • Yujie Ding ,
  • Ruqin Yu
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  • a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
    b College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
    c National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs, Beijng 100700, China
    d School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China
    e Beijing Tongrentang Pingjiang Atractylodes macrocephala Koidz Co., Ltd, Pingjiang 414500, China

Received date: 2021-09-27

  Online published: 2021-12-29

Supported by

National Key R&D Program of China(2020YFC1712700); Scientific Research Project of the Education Department of Hunan Province(19C0554)

摘要

本研究主要采用电感耦合等离子体质谱仪(ICP-MS)等手段测定5个产地白术41种矿质元素含量和土壤41种化学成分指标, 结合聚类分析法、偏最小二乘判别分析(PLS-DA)法和Pearson相关性分析研究了不同产区白术矿质元素特征以及不同产区土壤因子的差异, 并对它们的相关性进行了探讨. 结果表明, 采用无监督的聚类分析和有监督的PLS-DA均能正确判别不同产地的白术和土壤, 其中稀土元素在浙江白术与土壤间呈正相关. 另外, Li、V、Mn、Co、Cu、Rb、Cd、Cs、Ba等微量元素是白术产地溯源的关键因素. 研究发现在白术生产实践中可以通过适量控制种植地的土壤湿度、增施特定肥料等措施调控白术矿质元素含量. 本研究将为解释道地白术的品质形成机制, 保证白术药效质量的有效性提供一定帮助.

本文引用格式

王小芝 , 陈瑶 , 吴海龙 , 王童 , 杨健 , 付海燕 , 杨小龙 , 李旭富 , 丁玉洁 , 俞汝勤 . 基于元素指纹的白术产地溯源及其与土壤的相关性研究[J]. 化学学报, 2022 , 80(2) : 159 -167 . DOI: 10.6023/A21090441

Abstract

We explored the characteristics of mineral elements of Atractylodes macrocephala Koidz. (AMK) in different producing areas and their correlation with soil factors, which is expected to provide some guidance for the artificial cultivation of AMK. The test solutions of AMK and soil were prepared by digestions, AMK digested with HNO3, while soil using mixed acid such as HClO4+H2SO4+HF+HNO3. In this study, the content of 41 mineral elements in AMK and 41 chemical composition indexes in soil from 5 regions in china were mainly determined by inductively coupled plasma mass spectrometry (ICP-MS). Among them, the chemical composition of Na2O, MgO, Al2O3, K2O, CaO, TFe2O3, Ba, Cr, Mn, Ni, Sr, V in soil were measured by inductively coupled plasma optical emission spectrometry (ICP-OES), and As, Se were detected by atomic fluorescence spectrometry (AFS). In addition, one-way analysis of variance (ANOVA) was used to analyze the difference of mineral element content in different regions, cluster analysis and partial least squares discriminant analysis (PLS-DA) were used for pattern recognition, Pearson correlation analysis was applied to discuss the relationship between multi-element in AMK and soil factors. The results show that both the unsupervised cluster analysis and the supervised PLS-DA can correctly distinguish AMK and soil from different habitats. The content of rare earth elements in AMK in Zhejiang Province is positively correlated with the content of rare earth elements in soil, which also affected by other mineral elements in the soil. Besides, trace elements like Li, V, Mn, Co, Cu, Rb, Cd, Cs, Ba can be regarded as the key factors for traceability of AMK. K2O, CaO, Na2O, MgO, Sr, Mn, Co, Cr, Se, Ni, Cd can be used as the basis for soil discrimination. Mineral elements can be regulated by appropriate control of soil moisture and application of specific fertilizer in the production practice of AMK. Moreover, soil is an important factor for the formation of genuine medicinal materials. Rare earth elements in the soil can promote the absorption of Mn, Zn and other trace elements in AMK. This study will provide some help and references to explain the formation mechanism of genuine AMK and ensure its efficacy and quality.

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