1 引言
2 结果与讨论
2.1 探针SBODFN和DBODFN对H2S的荧光响应特性研究
图4 (a)探针SBODFN在HEPES缓冲液中与不同分析物反应36 min后在593 nm处的荧光强度; (b)探针DBODFN在HEPES缓冲液中与不同分析物反应42 min后在663 nm处的荧光强度Figure 4 (a) Fluorescence intensity of SBODFN at 593 nm with the addition of different substances for 36 min in HEPES buffer; (b) Fluorescence intensity of DBODFN at 663 nm with the addition of different substances for 42 min in HEPES buffer |
图6 (a)探针SBODFN在HEPES缓冲液中与不同浓度的H2S孵育36 min后的荧光发射光谱; (b)探针SBODFN在HEPES缓冲液中与不同浓度的H2S孵育36 min后在593 nm处荧光强度线性拟合图Figure 6 (a) Fluorescence emission spectra of SBODFN upon the addition of H2S at varying concentrations for 36 min in HEPES buffer; (b) Linear fitting plot of fluorescence intensity at 593 nm for SBODFN incubated with H2S at varying concentrations for 36 min in HEPES buffer |
图7 (a)探针DBODFN在HEPES缓冲液中与不同浓度的H2S孵育42 min后的荧光发射光谱; (b)探针DBODFN在HEPES缓冲液中与不同浓度的H2S孵育42 min后在663 nm处荧光强度线性拟合图Figure 7 (a) Fluorescence emission spectra of DBODFN upon the addition of H2S at varying concentrations for 42 min in HEPES buffer; (b) linear fitting plot of fluorescence intensity at 663 nm for DBODFN incubated with H2S at varying concentrations for 42 min in HEPES buffer |
图8 SBODFN在HEPES缓冲液中(a)在不同温度条件下与(b)在不同pH下H2S孵育36 min后593 nm处的荧光强度Figure 8 Fluorescence intensity of SBODFN (593 nm) incubated with H2S for 36 min under (a) different temperature and (b) different pH in HEPES buffer |
2.2 探针SBODFN和DBODFN对H2S的紫外-可见吸收响应特性研究
图10 (a) SBODFN在HEPES缓冲液中与不同浓度的H2S孵育36 min后的紫外-可见吸收光谱; (b) DBODFN在HEPES缓冲液中与不同浓度的H2S孵育42 min后的紫外-可见吸收光谱Figure 10 (a) UV-vis absorption spectra of SBODFN incubated with varying concentrations of H2S for 36 min in HEPES buffer; (b) UV-vis absorption spectra of DBODFN incubated with varying concentrations of H2S for 42 min in HEPES buffer |
2.3 SBODFN和DBODFN检测H2S的机理探索
图11 (a) SBODFN加入H2S后和SBODOH的紫外-可见吸收光谱; (b) SBODFN加入H2S后和SBODOH的荧光发射光谱Figure 11 (a) UV-vis absorption spectra of SBODFN added with H2S and SBODOH; (b) fluorescence emission spectra of SBODFN added with H2S and SBODOH |
2.4 SBODFN和DBODFN作为固体探针的研究
2.5 SBODFN和DBODFN在实际水样上的检测
表1 SBODFN对不同水样中H2S的检测Table 1 Detection of H2S in different water samples by SBODFN |
| Sample | Added/(μmol•L-1) | Fluorescence Intensity | Measured Value /(μmol•L-1) | Recovery/% | RSD/% |
|---|---|---|---|---|---|
| Tap water | 380 | 1858 | 406 | 106.82 | 4.89 |
| 1720 | 370 | 97.26 | |||
| 1828 | 398 | 104.74 | |||
| lake water | 380 | 1817 | 395 | 103.98 | 6.33 |
| 1683 | 360 | 94.70 | |||
| 1862 | 407 | 107.09 | |||
| Yellow River water | 380 | 1689 | 361 | 95.11 | 2.94 |
| 1764 | 381 | 100.31 | |||
| 1697 | 364 | 95.67 |
表2 DBODFN对不同水样中H2S的检测Table 2 Detection of H2S in different water samples by DBODFN |
| Sample | Added/(μmol•L-1) | Fluorescence Intensity | Measured Value /(μmol•L-1) | Recovery/% | RSD/% |
|---|---|---|---|---|---|
| Tap water | 440 | 1781 | 448 | 101.73 | 2.50 |
| 1734 | 440 | 99.90 | |||
| 1654 | 426 | 96.80 | |||
| lake water | 440 | 1761 | 444 | 100.95 | 0.85 |
| 1804 | 452 | 102.62 | |||
| 1773 | 446 | 101.42 | |||
| Yellow River water | 440 | 1823 | 455 | 103.36 | 3.83 |
| 1636 | 423 | 96.10 | |||
| 1787 | 449 | 101.96 |