Research Progress on Surface-Enhanced Raman Spectroscopy Technique for the Detection of microRNA

doi:10.6023/A21010017

Acta Chimica Sinica ›› 2021, Vol. 79 ›› Issue (6): 694-704.DOI: 10.6023/A21010017 Previous Articles Next Articles

Special Issue: 分子探针、纳米生物学与生命分析化学

Review

表面增强拉曼光谱技术在microRNA检测中的研究进展

易荣楠^a, 吴燕^b^,^*()

a 湖南警察学院刑事科学技术系长沙 410138
b 长江师范学院化学化工学院重庆 408100

投稿日期:2021-01-19 发布日期:2021-03-30
通讯作者: 吴燕

作者简介:

易荣楠, 男, 1992年生, 主要从事拉曼光谱技术在毒物毒品中的分析检验、有机合成方法学等方面的研究. 作为核心成员参与国家自然科学基金项目2项, 发表SCI论文8篇.

吴燕副教授, 女, 1988年生, 主要研究方向为SERS技术及生物传感器的应用研究. 主持国家自然科学基金、重庆市科委项目、重庆市教委项目、长江师范学院青年人才成长支持计划项目、长江师范学院科研启动项目等6项. 作为骨干成员参与多项国家自然科学基金、重庆市自然科学基金等项目的研究. 近年来, 以第一作者与通讯作者发表SCI论文12篇, 授权发明专利1项, 发表教改论文2篇.

基金资助:
国家自然科学基金(21804011)

Research Progress on Surface-Enhanced Raman Spectroscopy Technique for the Detection of microRNA

Rongnan Yi^a, Yan Wu^b()

a Criminal Technology Department, Hunan Police Academy, Changsha 410138, China
b College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China

Received:2021-01-19 Published:2021-03-30
Contact: Yan Wu
Supported by:
National Natural Science Foundation of China(21804011)

microRNA is an endogenous non-coding single-stranded RNA with a length of about 18~24 nucleotides. The latest research has found that the occurrence of many diseases and tumors is closely related to the level of microRNA expression, and microRNA is expected to become a new tumor marker and a new target for cancer treatment. Therefore, the development of high sensitivity, high specificity, and simple and rapid microRNA detection methods is greatly significant for biomedical research and early diagnosis of cancer. Surface-enhanced Raman spectroscopy (SERS) technique has great application value in the field of early cancer diagnosis due to its unique advantages such as high sensitivity, fast detection speed, fingerprint recognition, and low water interference. The latest research progress of SERS technique in microRNA detection is summarized in this review. Finally the main challenges of SERS technique in bioassay are discussed and the future development trend is proposed.

Key words: surface-enhanced Raman spectroscopy, microRNA, tumor marker

Cite this article

Rongnan Yi, Yan Wu. Research Progress on Surface-Enhanced Raman Spectroscopy Technique for the Detection of microRNA[J]. Acta Chimica Sinica, 2021, 79(6): 694-704.

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Fig. & Tab. 14

Figure 1. Schematic illustration of a synergistic calibrated SERS strategy for the detection of cancer-related microRNA-182[17]

Figure 2. Schematic for SERS biosensor based on Fe3O4@PDA/Pt and DSN signal amplification for microRNA-155 detection[19]

Figure 3. (A) Schematic representation of Y-DNA-driven construction of Au NP chiral C30S5S10 NSs with enhanced CD and SERS signals for the detection of microRNA in tumor cell[25]; (B) illustration of the synthesis of CuPc@HG@BN probe and the antitumor and SERS monitoring mechanism[26]

Figure 4. Schematic of DNA-mediated gold-silver nano-mushrooms as SERS probes for ultrasensitive and multiplex DNA/miRNA detection[27]

Figure 5. Schematic of the AgNP dimer probes and the protocol for microRNA-21 detection with the in situ hot-spot assembly strategy[33]

Figure 6. Schematic of the optical sensor based on target microRNA-directed formation of AuNR chiral side-by-side dimers for the detection of intracellular microRNA[36]

Figure 7. Schematic illustration of the DNA-mediated SERS probe for multiplex microRNA detection[40]

Figure 8. Schematic of SERS sensing platform for microRNA detection via microRNA-triggered hybridization chain reaction and Ag+-mediated cascade amplification [50]

Figure 9. Schematic of SERS sensor for microRNA-155 detection based on padlock probe-based exponential rolling circle amplification strategy[52]

Figure 10. Schematic of dual-mode imaging and reverse signal ratio quantification of microRNA-203 in cells with BSR probes[66]

Figure 11. Schematic diagram of duple signal amplification mechanism based SERS-microfluidics platform for determination of microRNA-21[68]

Figure 12. Schematic illustrations of principle of the SERS-based lateral flow sensing strategy coupled with CHA for the detection of microRNA-21[71]

Figure 13. Schematic illustrations of (A) principle of Au-UCNP pyramids for microRNA detection and (B) the nucleic acid skeleton of pyramid used for microRNA detection[74]

Figure 14. Schematic illustrations of principle of the SERS-based sandwich biosensor coupled with Ag/TiO2 NWs for the detection of microRNA-21[78]

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表面增强拉曼光谱技术在microRNA检测中的研究进展

Research Progress on Surface-Enhanced Raman Spectroscopy Technique for the Detection of microRNA

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