Acta Chimica Sinica ›› 2022, Vol. 80 ›› Issue (2): 159-167.DOI: 10.6023/A21090441 Previous Articles     Next Articles



王小芝a, 陈瑶a,b,*(), 吴海龙a, 王童a, 杨健c, 付海燕d, 杨小龙d, 李旭富e, 丁玉洁a,*(), 俞汝勤a   

  1. a 化学生物传感与计量学国家重点实验室 湖南大学化学化工学院 长沙 410082
    b 湖南工业大学 生命科学与化学学院 株洲 412007
    c 中国中医科学院道地药材国家重点实验室培育基地 国家中药资源中心 北京 100700
    d 中南民族大学 药学院 武汉 430074
    e 北京同仁堂平江白术有限公司 平江 414500
  • 投稿日期:2021-09-27 发布日期:2021-12-28
  • 通讯作者: 陈瑶, 丁玉洁
  • 基金资助:
    国家重点研发计划(2020YFC1712700); 湖南省教育厅科学研究项目(19C0554)

Study on the Origin Traceability of Atractylodes macrocephala Koidz. and Its Correlation with Soil Based on Mineral Elements

Xiaozhi Wanga, Yao Chena,b(), Hailong Wua, Tong Wanga, Jian Yangc, Haiyan Fud, Xiaolong Yangd, Xufu Lie, Yujie Dinga(), Ruqin Yua   

  1. a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
    b College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
    c National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs, Beijng 100700, China
    d School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China
    e Beijing Tongrentang Pingjiang Atractylodes macrocephala Koidz Co., Ltd, Pingjiang 414500, China
  • Received:2021-09-27 Published:2021-12-28
  • Contact: Yao Chen, Yujie Ding
  • Supported by:
    National Key R&D Program of China(2020YFC1712700); Scientific Research Project of the Education Department of Hunan Province(19C0554)

We explored the characteristics of mineral elements of Atractylodes macrocephala Koidz. (AMK) in different producing areas and their correlation with soil factors, which is expected to provide some guidance for the artificial cultivation of AMK. The test solutions of AMK and soil were prepared by digestions, AMK digested with HNO3, while soil using mixed acid such as HClO4+H2SO4+HF+HNO3. In this study, the content of 41 mineral elements in AMK and 41 chemical composition indexes in soil from 5 regions in china were mainly determined by inductively coupled plasma mass spectrometry (ICP-MS). Among them, the chemical composition of Na2O, MgO, Al2O3, K2O, CaO, TFe2O3, Ba, Cr, Mn, Ni, Sr, V in soil were measured by inductively coupled plasma optical emission spectrometry (ICP-OES), and As, Se were detected by atomic fluorescence spectrometry (AFS). In addition, one-way analysis of variance (ANOVA) was used to analyze the difference of mineral element content in different regions, cluster analysis and partial least squares discriminant analysis (PLS-DA) were used for pattern recognition, Pearson correlation analysis was applied to discuss the relationship between multi-element in AMK and soil factors. The results show that both the unsupervised cluster analysis and the supervised PLS-DA can correctly distinguish AMK and soil from different habitats. The content of rare earth elements in AMK in Zhejiang Province is positively correlated with the content of rare earth elements in soil, which also affected by other mineral elements in the soil. Besides, trace elements like Li, V, Mn, Co, Cu, Rb, Cd, Cs, Ba can be regarded as the key factors for traceability of AMK. K2O, CaO, Na2O, MgO, Sr, Mn, Co, Cr, Se, Ni, Cd can be used as the basis for soil discrimination. Mineral elements can be regulated by appropriate control of soil moisture and application of specific fertilizer in the production practice of AMK. Moreover, soil is an important factor for the formation of genuine medicinal materials. Rare earth elements in the soil can promote the absorption of Mn, Zn and other trace elements in AMK. This study will provide some help and references to explain the formation mechanism of genuine AMK and ensure its efficacy and quality.

Key words: Atractylodes macrocephala Koidz., soil factors, mineral elements, quantitative analysis, geographic origin, correlation analysis