One new flavonoside and four known compounds were separated from the water soluble fractions of seed of Oxytropis ochrocephala Bunge by using semi-preparative HPLC. Four known compounds have been identified as 2-methyl-3-hydroxy-4H-Pyran-4-one, rhamnocitrin-3-O-β -D-galaetoside-4'-O-β-D-glucoside, rhamnocitrin-3-O-β -D- galactoside, and rhamnocitrin-3-O-β-D-galactoside. The structure of new flavonoside, as 5-methoxy-7-hydroxy-3-O-β -D-galactoside-4'-O-β- D-glucoside, has been established on the basis of HPLC retention times and spectral evidences.

Key words: CHEMICAL COMPOSITION, SEED, HPLC, INFRARED SPECTROPHOTOMETRY, ULTRAVIOLET SPECTROPHOTOMETRY, STRUCTURE, MASS SPECTROGRAPHY