A new chiral stationary phase, heptakis(2,6-di-O-n-butyl-3-O-trifluoroacetyl)-b-cyclodextrin (DB-TFA-b-CD), has been prepared from native b-cyclodextrin, and coated on fused silica capillary column. The sepns. of optical isomers including halohydrocarbon, epoxy compounds, ketones, alcs., diols, amines, carboxylic acids, and amino acids have been achieved. The results showed that the acyl groups of alcohol derivatives played a significant role in their enantioselective sepns. The choice of an acylation reagent can mean the difference between success and failure in obtaining an enantiomeric separation of alcohol Iso-leucine, allo-isoleucine, and proline which were seriously overlapped or poorly separated by amide CSPs were base-lined resolved on this CSP.

Key words: GAS CHROMATOGRAPHY, STATIONARY PHASE, CYCLODEXTRIN, QUARTZ, CHIRALITY, OPEN TUBULAR COLUMN