The immobilization of penicillin G amidase on mesoporous  molecular sieve MCM-41

Acta Chimica Sinica ›› 2000, Vol. 58 ›› Issue (2): 167-171. Previous Articles Next Articles

Original Articles

青霉素酰化酶在介孔分子筛MCM-41上的固定化研究

李晓芬;何静;段雪;马润宇;朱月香

北京化工大学应用化学系.北京(100029);北京化工大学化学工程系;北京大学物理化学研究所.北京(100871)

发布日期:2000-02-15

The immobilization of penicillin G amidase on mesoporous molecular sieve MCM-41

Li Xiaofen;He Jing;Duan Xue;Ma Runyu;Zhu Yuexiang

Beijing Univ Chem Technol, Dept Appl Chem.Beijing(100029);Beijing Univ, Inst Phys Chem.Beijing(100871)

Published:2000-02-15

Abstract

Mesoporous molecular sieve MCM-41 has been used as the support for the immobilization of penicillin G amidase (PGA). The effects of immobilization conditions such as pH, temperature, time and ratio of PGA to MCM-41 on the activity and stability of immobilized enzyme (IME) have been studied. It was found that the activity of IME decreased with the increase of pH, increased and then decreased with the increase of temperature and time, increased with the increase of the ratio of PGA to MCM-41. It was also found that the stability of IME increased with the increase of temperature, increased and then decreased with the increase of time and the ratio of PGA to MCM-41. Additionally, the process of PGA immobilized on MCM-41 has been studied.

Key words: MOLECULAR SIEVE, FIXATION

CLC Number:

Cite this article

Li Xiaofen;He Jing;Duan Xue;Ma Runyu;Zhu Yuexiang. The immobilization of penicillin G amidase on mesoporous molecular sieve MCM-41[J]. Acta Chimica Sinica, 2000, 58(2): 167-171.

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青霉素酰化酶在介孔分子筛MCM-41上的固定化研究

The immobilization of penicillin G amidase on mesoporous molecular sieve MCM-41

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