Capillary electrophoresis was developed to determine excitatory amino acids in cerebrospinal fluid following cerebral injury. The effect of injection module, concentration of buffer, organic additive, and derivative conditions on determination minute quantity of excitatory amino acids in cerebrospinal fluid was investigated. The limitation of detection was less than 1.7 * 10~(-7)mol/L under the optimum conditions with benzene carboxylic acid as an internal standard. The reproducibility was also satisfactory with the relative standard deviation less than 2%.

Key words: ELECTROPHORESIS, AMINO ACID, CEREBRO-SPINAL FLUID