Direct Electrochemistry of Horseradish Peroxidase at a Carbon Nanotube Electrode

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (3): 335-340. Previous Articles Next Articles

碳纳米管电极上辣根过氧化物酶的直接电化学

蔡称心, 陈静

南京师范大学化学系, 南京, 210097

投稿日期:2003-09-15 修回日期:2003-11-29 发布日期:2014-01-26
通讯作者: 蔡称心,E-mail:cxcai@njnu.edu.cn. E-mail:cxcai@njnu.edu.cn
基金资助:
国家自然科学基金(No.20373027)、教育部留学回国人员启动基金、江苏省高校自然科学基金(No.03KJA150055)和江苏省南京市人事局回国人员择优项目资助课题.

Direct Electrochemistry of Horseradish Peroxidase at a Carbon Nanotube Electrode

CAI Chen-Xin, CHEN Jing

Department of Chemistry, Nanjing Normal University, Nanjing 210097

Received:2003-09-15 Revised:2003-11-29 Published:2014-01-26

Abstract

Horseradish peroxidase (HRP) immobilized on the surface of the carbon nanotube modified glassy carbon electrode (HRP-CNT/GC) can undergo effective and stable direct electron transfer reaction. The cyclic voltammetric results indicate that the HRP-CNT/GC electrode exhibits a pair of well-defined and nearly symmetrical redox peaks in 0.1 mol/L phosphate buffer solution. Its formal redox potential, E^0', is almost independent on the scan rates, the average value of E^0' is (-0.319±0.002) V (vs. SCE, pH 6.9) in the phosphate buffer solution (pH 6.9) in the scan rate range of 20～100 mV/s. The dependence of E^0' on the pH of the buffer solution indicates that the direct electron transfer of HRP is a one-electron-transfer reaction process coupled with one-proton-transfer. The experimental results also demonstrated that the immobilized HRP retained its bioelectrocatalytic activity for the reduction of H₂O₂ and the HRP-CNT/GC electrode can rapidly respond to the change of the concentration of H₂O₂. The method presented here can be easily extended to obtain the direct electrochemistry of other redox enzymes or proteins.

Key words: carbon nanotube, chemically modified electrode, direct electrochemistry, horseradish peroxidase

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CAI Chen-Xin, CHEN Jing. Direct Electrochemistry of Horseradish Peroxidase at a Carbon Nanotube Electrode[J]. Acta Chimica Sinica, 2004, 62(3): 335-340.

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碳纳米管电极上辣根过氧化物酶的直接电化学

Direct Electrochemistry of Horseradish Peroxidase at a Carbon Nanotube Electrode

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