To combine the advantages of high-throughput microarray and microfluidic chip technology, microarray was fabricated in channels. DNA and protein were arrayed on quartz slide, which was reversibly sealed by poly(dimethylsiloxane) (PDMS) slab containing pattern of channels. The hybridization carried out in channels indicated that target DNA and proteins were bound to their complementary DNA and protein probes specifically. In this method, PDMS conjugated with polylysine was contributed to imparting the wettability and decreasing unspecific adsorption of biomolecules. Our data demonstrated that the integrated chip speeded up the detection compared to microarrays, extended the parallel channels with serial properties and enhanced the throughput of probes and targets.

Key words: microarray, microfluidic chip, modification, polylysine