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Acta Chimica Sinica ›› 2006, Vol. 64 ›› Issue (6): 537-542. Previous Articles     Next Articles

一种基于电洗脱的核酸纯化回收芯片的设计及优化

冉瑞，李刚，赵辉，刘康栋，金庆辉，杨梦苏，赵建龙*   

  1. (1中国科学院上海微系统与信息技术研究所 上海 200050)
    (2中国科学院研究生院 北京100049)
    (3生物芯片上海国家工程研究中心 上海 201203)
  • 投稿日期:2005-07-12 修回日期:2005-11-30 发布日期:2006-03-28
  • 通讯作者: 赵建龙

Design and Optimization of a Chip Based on Electroelution for the Purification and Recovery of DNA Fragments

RAN Rui, LI Gang, ZHAO Hui, LIU Kang-Dong, JIN Qing-Hui, YANG Meng-Su, ZHAO Jian-Long*   

  1. (1 Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050)
    (2 Graduate School of Chinese Academy of Sciences, Beijing 100049)
    (3 National Engineering Center for Biochip at Shanghai, Shanghai 201203)
  • Received:2005-07-12 Revised:2005-11-30 Published:2006-03-28
  • Contact: ZHAO Jian-Long

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A chip based on electroelution principle was presented for the recovery of DNA fragments, which can make the isolation and collection of target DNA fragments possible in a single process by switching electrodes. An optimization design was made for the channel structure and the distribution of the electric field in DNA recovery chip, and experiments were also performed to validate the simulation result. Both theoretical analysis and experimental results show that the optimization design in our existing device can achieve the desired results.

Key words: nucleic acid, purification, recovery, numerical simulation