A novel cucurbit[6]uril monorotaxane bonded silica stationary phase (CB6MRBS) for high performance liquid chromatography has been prepared successfully by combining supramolecular self-assembly technology with the immobilization technique of chromatography stationary phase. A cucurbit[6]uril monorotaxane (CB6MR) was, for the first time, grafted to silica gel by using γ-glycidoxypropyltrimethoxysilane (KH-560) as coupling reagent. CB6MRBS was characterized by FTIR, elemental analysis and thermal analysis. The chromatographic performance and retention mechanism of the new packing material were evaluated by using organic components including acidic, basic, neutral analytes and positional isomers of disubstituted benzene as probe molecules under reversed-HPLC and normal-HPLC mobile phases. The results show that CB6MRBS is a sort of multimode bonded stationary phase exhibits excellent reversed-phase and normal-phase chromatographic property. CB6MRBS shows obvious priority for separation of positional isomers, especially for the efficient separation of basic compounds. The new packing can provide various action sites for different analytes, such as hydrogen-bonding, π-π, electrostatic and hydrophobic interactions, etc. It may have good prospect for complexation chromatography, which is required to further stduy.

Key words: high performance liquid chromatography, stationary phase, monorotaxane, cucurbit[6]uril