Immobilization, Characterization of DNA on the Surface of Carbon Nanotube and Electrochemical Detection of DNA Damage

Acta Chimica Sinica ›› 2008, Vol. 66 ›› Issue (4): 424-432. Previous Articles Next Articles

Original Articles

DNA在碳纳米管表面的固定、表征及损伤的电化学检测

刘姝娜, 吴萍, 周泊, 周耀明, 蔡称心*

(南京师范大学化学与环境科学学院生物功能材料江苏省重点实验室
分子医学生物技术江苏省重点实验室南京 210097)

投稿日期:2007-07-11 修回日期:2007-09-21 发布日期:2008-02-28
通讯作者: 蔡称心

Immobilization, Characterization of DNA on the Surface of Carbon Nanotube and Electrochemical Detection of DNA Damage

LIU Shu-Na; WU Ping; ZHOU Bo; ZHOU Yao-Ming; CAI Chen-Xin*

(College of Chemistry and Environmental Science, Jiangsu Key Laboratory for Biofunctional Materials, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Nanjing Normal University, Nanjing 210097)

Received:2007-07-11 Revised:2007-09-21 Published:2008-02-28
Contact: CAI Chen-Xin

Abstract

DNA-PDDA-CNT nanocomposites were fabricated by immobilizing DNA on the surface of single-walled carbon nanotubes (CNT) via poly(dimethyldiallylammonium chloride) (PDDA). The atomic force microscopy (AFM), ultraviolet-visible (UV-Vis), Raman spectroscopy and impedance spectroscopy (EIS) were used to characterize the growth processes of the nanocomposites. The cyclic voltammetric results indicate that the interaction between 1-phenylazo-2-naphthol (PN) and the immobilized DNA is intercalative binding, and PN can be used as a probe for detection of DNA damage. PN seems to be an attractive probe for DNA damage analysis since its redox peak is at ca. 0.1 V (vs. SCE, pH 5.5), where interference should be minimal.

Key words: carbon nanotube, DNA, DNA damage, electrochemical detection

Cite this article

LIU Shu-Na; WU Ping; ZHOU Bo; ZHOU Yao-Ming; CAI Chen-Xin*. Immobilization, Characterization of DNA on the Surface of Carbon Nanotube and Electrochemical Detection of DNA Damage[J]. Acta Chimica Sinica, 2008, 66(4): 424-432.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

[1]	Tiancheng Zhao, Hongyu Jiang, Kun Zhang, Yifan Xu, Xinyue Kang, Jiancheng Xu, Xufeng Zhou, Peining Chen, Huisheng Peng. Continuous Preparation of High-performing Carbon Nanotube Fibers Based on Cycloalkane/ethanol Mixing Carbon Source [J]. Acta Chimica Sinica, 2023, 81(6): 565-571.
[2]	Lanying Li, Qing Tao, Yanli Wen, Lele Wang, Ruiyan Guo, Gang Liu, Xiaolei Zuo. Poly-adenine-based DNA Probes and Their Applications in Biosensors^★ [J]. Acta Chimica Sinica, 2023, 81(6): 681-690.
[3]	Wen Liu, Yujie Wang, Huiqin Yang, Chengjie Li, Na Wu, Yang Yan. The Preparation of Carbon Nanotubes/Reduced Graphene Oxide Current Collector by Non-covalent Induction of Ionic Liquid for Sodium Metal Anode [J]. Acta Chimica Sinica, 2023, 81(10): 1379-1386.
[4]	Shouming Cheng, Bo Zhou. Molecular Insights into the Transformation Mechanisms of Dissolved Organic Matter Based on Ultrahigh Resolution Mass Spectrometry [J]. Acta Chimica Sinica, 2022, 80(8): 1106-1114.
[5]	Chaofeng Wang, Guodong Zheng, Yue Wang, Huijia Song, Xiaoyi Chen, Ruixia Gao. Preparation of Controllable Non-covalent Functionalized Carbon Nanotubes with Metalloporphyrin-Sn Network and Application to Protein Adsorption [J]. Acta Chimica Sinica, 2022, 80(2): 126-132.
[6]	Yijun Guo, Bing Wei, Xiang Zhou, Dongbao Yao, Haojun Liang. DNA Walker-Programmed Nanoparticle Superlattice [J]. Acta Chimica Sinica, 2021, 79(2): 192-199.
[7]	Zhu Qingqing, Song Xiaojun, Deng Zhaoxiang. Tunable Charge Transfer Plasmon at Gold/Copper Heterointerface [J]. Acta Chimica Sinica, 2020, 78(7): 675-679.
[8]	Li Haimei, Luo Huajian, Xiao Qi, Yang Liyun, Huang Shan, Liu Yi. Investigations of Interactions and Mechanisms of Chiral Graphene Quantum Dots with DNA [J]. Acta Chimica Sinica, 2020, 78(6): 577-586.
[9]	Liu Qiyan, Cai Daihong, Qi Yongyu, Le Xueyi. DNA Interaction and Antitumor Activity of A Copper(II) Complex Containing Sparfloxacin and Triazine Derivatives [J]. Acta Chimica Sinica, 2020, 78(3): 263-270.
[10]	Zhao Li-Dong, Zuo Peng, Yin Bin-Cheng, Hong Chenglin, Ye Bang-Ce. A Cell Membrane-Anchored DNA Tetrahedral Sensor for Real-time Monitoring of Exosome Secretion [J]. Acta Chimica Sinica, 2020, 78(10): 1076-1081.
[11]	Chi Jingyuan, Li Jing, Ren Shaokang, Su Shao, Wang Lianhui. Construction and Application of DNA-two-dimensional Layered Nanomaterials Sensing Platform [J]. Acta Chimica Sinica, 2019, 77(12): 1230-1238.
[12]	Yang, Wei-Yu, Lei, Zhi-Chao, Hong, Wenjing, Huang, Fei-Zhou. Advances in Charge Transport through DNA Molecular Junction by Employing Electrodes Pair with Nanometer-sized Separation [J]. Acta Chimica Sinica, 2019, 77(10): 951-963.
[13]	Cui Lirui, Zhang Jin, Sun Yiyan, Lu Shanfu, Xiang Yan. Effect of Addition of Carbon Nanotubes on the Performance of a Low Pt Loading Membrane-Electrode-Assembly in Proton Exchange Membrane Fuel Cells [J]. Acta Chim. Sinica, 2019, 77(1): 47-53.
[14]	Huang Wenjiao, Zhang Haoyu, Hu Shuozhen, Niu Dongfang, Zhang Xinsheng. Effect of Nitrogen-Containing Functional Groups of Cobalt Phthalocyanine Catalyst on the Oxygen Reduction Performance in Fuel Cells [J]. Acta Chim. Sinica, 2018, 76(9): 723-728.
[15]	Zhu Mingjing, Peng Juan, Tang Ping, Qiu Feng. Preparation and Characterization of Highly Stable and Aqueous Dispersion of Conjugated Polyelectrolyte/Single-Walled Carbon Nanotube Nanocomposites [J]. Acta Chim. Sinica, 2018, 76(6): 453-459.

DNA在碳纳米管表面的固定、表征及损伤的电化学检测

Immobilization, Characterization of DNA on the Surface of Carbon Nanotube and Electrochemical Detection of DNA Damage

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments