In the paper, a novel chemiluminescence-flow injection method was proposed for determination of trace dopamine which is based on an ultrasensitive CL system of luminol-H2O2-diperiodatocuprate coupled with a selective enzymatic reaction in a potato tissue. The linear range of calibration curve was 2.1×10-10 mol L-1 to 2.1×10-6 mol L-1 and the limit detection was 7.2×10-11 mol L-1 .The relative standard deviation for 3.0×10-9 mol L-1 of dopamine was 2.1% (n=7). The proposed method was successfully applied for the direct determination of inherent dopamine in human serum.

Key words: chemiluminescence, diperiodatocuprate, potato tissue, dopamine