A Novel Method to Detect Muramidase Released Protein(MRP)  Antigen of Streptococcus sui Serotype 2 Based on  CdSe/ZnS Quantum Dot Probe

Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (10): 1087-1092. Previous Articles Next Articles

Original Articles

CdSe/ZnS量子点探针用于检测猪链球菌2型溶菌酶释放蛋白(MRP)抗原的新方法研究

武红敏a 韩鹤友*,a 金梅林b 张安定b

(a华中农业大学理学院农业微生物学国家重点实验室武汉 430070)
(b华中农业大学动物科技学院农业微生物学国家重点实验室武汉 430070)

投稿日期:2008-07-04 修回日期:2008-12-20 发布日期:2009-05-28
通讯作者: 韩鹤友

A Novel Method to Detect Muramidase Released Protein(MRP) Antigen of Streptococcus sui Serotype 2 Based on CdSe/ZnS Quantum Dot Probe

Wu, Hongmin ^a Han, Heyou ^*,a Jin, Meilin ^b Zhang, Anding ^b

(^aCollege of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070)
(^bCollege of Veterinary Medicine, State Key Laboratory of Agricultural Microbiology,
Huazhong Agricultural University, Wuhan 430070)

Received:2008-07-04 Revised:2008-12-20 Published:2009-05-28
Contact: Han, Heyou

Abstract

High-quality CdSe/ZnS quantum dot and the CdSe/ZnS quantum dot-muramidase released protein (MRP) antibody probe were prepared, and the conjugation mechanism was studied according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and molecular spectrometry. The factors influencing the preparation of CdSe/ZnS quantum dot-MRP antibody probe were optimized by fluorescence spectrometry. Under the optimum conditions, the probe showed good sensitivity to MRP antigen. The calibration graph was linear in the range from 5.0×10－8 to 1.5×10－6 mol/L of MRP antigen concentration, with a correlation coefﬁcient of 0.9976 and a detection limit of 1.9×10－8 mol/L.

Key words: Streptococcus Suis serotype 2, muramidase released protein (MRP), CdSe/ZnS quantum dot probe, detection

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CdSe/ZnS量子点探针用于检测猪链球菌2型溶菌酶释放蛋白(MRP)抗原的新方法研究

A Novel Method to Detect Muramidase Released Protein(MRP) Antigen of Streptococcus sui Serotype 2 Based on CdSe/ZnS Quantum Dot Probe

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