Five oligosaccharides were obtained from κ-carrageenan mild acid hydrolysate with gel filtration chromatography, which were linked to dipalmitoyl phosphatidylethanolamine (DPPE) by a reductive animation reaction to acquire five neoglycolipids. With a high-sensitivity electrospray ionization collision induced tandem mass spectrometry (ESI-CID-MS/MS) technique, the sequence and structure of these neoglycolipids were identified as κ-carrageenan derived trisaccharide, pentasaccharide, heptasaccharide, nonasaccharide and undecasaccharide-glycolipids, respectively. The results provide a good reference for synthesis of sulfated-neoglycolipid, and importantly, a basis for the preparation of the oligosaccharide-chip and the deep study on oligosaccharide-protein interactions.

Key words: κ-carrageenan oligosaccharide, neoglycolipid, synthesis, ESI-CID-MS/MS