Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (22): 2607-2612. Previous Articles     Next Articles

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N-磷酰化肽酯及小肽与溶菌酶相互作用的ESI-MS研究

强黎明a,b,董雪茹b,吕名秀b,曹书霞a,卢 奎*,b,赵玉芬*,a,c   

  1. (a郑州大学化学系 河南省化学生物学与有机化学重点实验室 郑州 450052) (b河南工程学院材料与化学工程系 郑州 450007) (c清华大学化学系 生命有机磷化学与化学生物学教育部重点实验室 北京 100084)
  • 投稿日期:2009-02-17 修回日期:2009-05-31 发布日期:2009-07-06
  • 通讯作者: 卢奎 E-mail:luckyluke@haut.edu.cn

Study on the Interaction between N-Phosphoryl Dipeptides (or Methyl Esters) and Lysozyme by ESI-MS

Qiang, Liming a,b,Dong, Xuerub,Lü, Mingxiub,Cao, Shuxiaa, Lu, Kui*,b,Zhao, Yufen*,a,c   

  1. (a Henan Province Key Laboratory of Chemical Biology and Organic Chemistry, Department of Chemistry, Zhengzhou University, Zhengzhou 450052) (b Department of Material and Chemical Engineering, Henan Institute of Engineering, Zhengzhou 450007) (c Educational Ministry Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084)
  • Received:2009-02-17 Revised:2009-05-31 Published:2009-07-06

The non-covalent interaction between a series of N-phosphoryl dipeptides (or methyl esters) and lysozyme was studied by using ESI-MS. The effects of different groups of N-phosphoryl dipeptides (or methyl esters) were compared. The results show that —OH is very important for the interaction, and the aromatic ring has double functions on the interaction because of its steric hindrance. The linked —OH and aromatic ring showed synergistic reaction and enhanced their interaction. Moreover, the size of the molecule and steric hindrance showed obvious effects on the interaction, while the configuration, sequence and length of carbon chains (increasing 1~2 C) of amino acid residue in the phosphoryl oligopeptides showed little effects on the interaction under ESI-MS conditions. Phosphoryl oligopeptides having extended construction, increasing molecular flexibility and smaller spatial hindrance could contract the protein configuration in solution, and the contractive protein molecule integrated with the smaller phosphoryl oligopeptide molecules more easily.

Key words: ESI-MS, N-phosphoryl dipeptide (or methyl esters), lysozyme, non-covalent interaction