Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (22): 2641-2644. Previous Articles    

Full Papers

时间分辨全反射红外光谱对醋酸诱导丝蛋白构象转变的研究

莫春丽a,DICKO Cedricb,邵正中*,a,陈新a   

  1. (a教育部聚合物分子工程重点实验室 复旦大学高分子科学系和先进材料实验室 上海 200433) (b牛津大学动物学系 牛津 OX3 3PS 英国)
  • 投稿日期:2009-04-27 修回日期:2009-05-21 发布日期:2009-07-08
  • 通讯作者: 邵正中 E-mail:zzshao@fudan.edu.cn
  • 基金资助:

    国家自然科学基金(No.20525414);科技部863计划(No.2006AA03Z452)

Time-Resolved Total Reflectance FT-IR Spectroscopy Characterization on Conformational Change of Silk Fibroin Induced by Acetic Acid

Mo, Chunlia,Dicko, Cedricb,Shao, Zhengzhong*,a,Chen, Xina   

  1. (a Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Advanced Materials Laboratory, Department of Macromolecular Science, Fudan University, Shanghai 200433) (b Oxford University, Zoology Department, South Parks Road, OX1 3PS, UK)
  • Received:2009-04-27 Revised:2009-05-21 Published:2009-07-08

It has been proved that time-resolved total reflectance FT-IR spectroscopy is a useful tool for investigating acetic acid diffusion in silk fibroin film and conformational transition of silk fibroin. Acetic acid could induce the construction of highly dominating content of β-sheet conformation in the silk fibroin film, in addition, the kinetic curve of organic solvent diffusion was similar to that of conformational transition as both processes reached equilibria at the same time. The access of acetic acid into the silk fibroin architecture appeared to be a necessary step in which the organic solvent could interact with silk protein, breaking its original hydrogen bond and inducing re-alignment of peptide chains. Furthermore, the content of water appeared to be an important factor in this dynamic system.

Key words: silk fibroin, acidity-basicity, diffusion, β-sheet, dynamic process