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Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (14): 1415-1420. Previous Articles     Next Articles

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用二维核磁共振研究[Co(phen)2(HNAIP)]Cl3配合物与错配及正常寡聚核苷酸的作用

席小莉1，杨曼曼2，陈绘丽1，杨频*,1   

  1. (1山西大学分子科学研究所化学生物学与分子工程教育部重点实验室 太原 030006)
    (2山西大学化学化工学院 太原 030006)
  • 投稿日期:2009-12-28 修回日期:2010-01-26 发布日期:2010-03-11
  • 通讯作者: 杨频 E-mail:yangpin@sxu.edu.cn

Study on the Interaction between Mismatched Oligonucleotide and Normal Oligonucleotide with [Co(phen)2(HNAIP)]Cl3 by 2D NMR

Xi Xiaoli1 Yang Manman2 Chen Huili1 Yang Pin*,1   

  1. (1 Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of China at College of Chemistry and Chemical Engineering, Institute of Molecular Science, Taiyuan 030006)
    (2 The College of Chemistry, Shanxi University, Taiyuan 030006)
  • Received:2009-12-28 Revised:2010-01-26 Published:2010-03-11

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In order to develop the probe that can identify mismatched oligonucleotide and normal oligonucleotide, we synthesized [Co(phen)2(HNAIP)]Cl3 metal complex firstly, and studied the interaction between mismatched oligonucleotide d(CCGAATGAGG)2 and normal oligonucleotide d(CCTAATTAGG)2 with [Co(phen)2(HNAIP)]Cl3 by 2D NMR. The results indicate that the complex binds the mismatched oligonucleotide by intercalation with the HNAIP ligand selectively inserted from the major groove between the stacked bases in the G3A4 and G7A8 region. So, it can identify G:A mismatch. At the same time, the complex binds the normal oligonucleotide by intercalation with the HNAIP ligand from the minor groove between the stacked bases in the terminal region. Because [Co(phen)2(HNAIP)]Cl3 can interact with mismatched oligonucleotide and normal oligonucleotide on different location and by different style, it can be used as a probe to identify mismatched oligonucleotide and normal oligonucleotide.

Key words: 2D NMR, oligonucleotide, DNA