Anodic electrochemiluminescence (ECL) of fluorescein, 2,7- dichlorofluorescein, tetrabromo-fluorescein and tetrachlorotetraiodofluorescein was found on the surface of platinum in the presence of sodium hydroxide. The effect of potential, pH and surfactants on the ECL properties were studied. It was found that there were also heavy atom effects in ECL. Fluorescence and ECL spectra of fluorescein were measured and the possible mechanism was proposed. Metal ions were examined and only copper (II) was found to enhance the ECL of fluorescein. Based upon it and combined with microdialysis sampling and anode stripping technique a new method to determine the metabolic process of copper in rabbit blood in vivo in high selectivity was established.

Key words: ELECTROCHEMISTRY, LUCIFERIN, PLATINUM, ELECTRODE, COPPER, ON-LINE ANALYSIS, CONCENTRATION