Amperometric Immunosensor Based on Mercapto Carboxylic Acid Self- assembled Monolayer for Phytohormone Indoleacetic Acid Assay

Acta Chimica Sinica ›› 2003, Vol. 61 ›› Issue (5): 790-794. Previous Articles Next Articles

Original Articles

基于巯基自组装单层膜的植物生长激素吲哚乙酸电化学免疫传感器的研究

李春香;李劲;萧浪涛;沈国励;俞汝勤

湖南大学化学化工学院;湖南农业大学

发布日期:2003-05-15

Amperometric Immunosensor Based on Mercapto Carboxylic Acid Self- assembled Monolayer for Phytohormone Indoleacetic Acid Assay

Li Chunxiang;Li Jin;Xiao Langtao;Shen Guoli;Yu Ruqin

State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University;Key Laboratory of Phytohormones, Hunan Agricultural University

Published:2003-05-15

Abstract

A competitive binding assay used to detennine hapten IAA with the aid of HRP-IAA conjugate has been developed. It is based on the ordered, stable mercapto self-assembled monolayer formed on gold surface, which immobilized IgG with covalent bond. The features of the substrate reaction under different conditions were investigated by cyclic voltammetry and the assay conditions were optimized. Under applied potential of - 200 mV, the response current is linear with lg [IAA], over a concentration range of 5.68 X 10-7 ~ 2.83 X 10-5 mol/L with a correlation coefficient of 0.9915. The sensor was applied for the determination of IAA sample with satisfactory results.

Key words: INDOLEACETIC ACID, CYCLOVOLTAMGRAPH, PLANT GROWTH REGULATORS, SENSORS, ELECTROCHEMISTRY

CLC Number:

TQ45

Cite this article

Li Chunxiang;Li Jin;Xiao Langtao;Shen Guoli;Yu Ruqin. Amperometric Immunosensor Based on Mercapto Carboxylic Acid Self- assembled Monolayer for Phytohormone Indoleacetic Acid Assay[J]. Acta Chimica Sinica, 2003, 61(5): 790-794.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

[1]	Xingyu Ma, Hui Sun, Jiang Li, Zhiyang Liu, Hongjun Zhou. “Continuous” Nitrogen Reduction Synthesis of Ammonia Based on Li-N₂ Battery System [J]. Acta Chimica Sinica, 2022, 80(7): 861-866.
[2]	Yao Zhai, Guoxiang Xin, Jiaqi Wang, Bangwen Zhang, Jinling Song, Xiaoxu Liu. Microwave-assisted Synthesis of rGO/CeO₂ Supercapacitor Electrode Materials with Excellent Electrochemical Properties [J]. Acta Chimica Sinica, 2021, 79(9): 1129-1137.
[3]	Hongyu Yuan, Minmin Xu, Jianlin Yao. SERS Studies on the Electrochemical and SPR Synergistic Catalytic Interfacial Reaction of 4-Chlorothiophenol [J]. Acta Chimica Sinica, 2021, 79(12): 1481-1485.
[4]	Liu Ji-Lin, Yu Kai, Zhang Hong, Jiang Jie. Progress in the Study of Electrochemical Reaction by Mass Spectrometric Ionization Sources [J]. Acta Chimica Sinica, 2020, 78(6): 504-515.
[5]	Yan Tingting, Xing Guolong, Ben Teng. One-step Strategy to Synthesize Porous Carbons by Carbonized Porous Organic Materials and Their Applications [J]. Acta Chim. Sinica, 2018, 76(5): 366-376.
[6]	Zhang Chengming, Pang Xin, Wang Yongzhao. Controllable Synthesis of One-dimensional Cryptomelane-type Manganese Dioxide and Its Electrochemical Performance [J]. Acta Chim. Sinica, 2018, 76(2): 133-137.
[7]	Yang Zheyao, Ma Wei, Ying Yilun, Long Yitao. Study on the Resolution of Single Silver Nanoparticles Electrochemical Behavior at Nanoelectrode [J]. Acta Chim. Sinica, 2017, 75(7): 671-674.
[8]	Dong Wei, Nie Mengsi, Lian Yongfu. Isolation and Electrochemical Property of Tb@C₈₂ Isomers [J]. Acta Chim. Sinica, 2017, 75(5): 453-456.
[9]	Zheng Yuan, Luo Jing, Wei Wei, Liu Xiaoya. Polyaniline-graphene Hollow Spheres based on Graphene Stabilized Pickering Emulsions [J]. Acta Chim. Sinica, 2017, 75(4): 391-397.
[10]	Yang Tao, Cui Yanan, Chen Huaiyin, Li Weihua. Controllable Preparation of Two Dimensional Metal- or Covalent Organic Frameworks for Chemical Sensing and Biosensing [J]. Acta Chim. Sinica, 2017, 75(4): 339-350.
[11]	Wang Yafeng, Yang Qian, Su Bin. Optical Sensors Based on Optical Interference of Nanoporous Film [J]. Acta Chim. Sinica, 2017, 75(11): 1071-1081.
[12]	Cui Xianghong, Chen Huaiyin, Yang Tao. Research Progress on the Preparation and Application of Nano-sized Molybdenum Disulfide [J]. Acta Chim. Sinica, 2016, 74(5): 392-400.
[13]	Li Laicai, Zhang Ming, Mao Shuang, Yang Chun, Tian Anmin. Theoretical Investigation on the Adsorption of DNA Bases on B-doped SWCNT Surface [J]. Acta Chimica Sinica, 2015, 73(2): 143-150.
[14]	Zhang Jiajia, Dai Peiqing, Li Chao, Li Nanwang, Cheng Guifang, He Pingang, Fang Yuzhi. A Symmetrically Split G-quadruplex DNAzymes Biosensor Based on Magnetic Nanoparticles for the Rapid Detection of Hg²⁺ [J]. Acta Chimica Sinica, 2014, 72(9): 1029-1035.
[15]	Lin Chao, Zhai Wei, Fan Louzhen, Li Xiaohong. DNA-Based Electrochemically Transduced and Resettable Keypad Lock [J]. Acta Chimica Sinica, 2014, 72(6): 709-712.

基于巯基自组装单层膜的植物生长激素吲哚乙酸电化学免疫传感器的研究

Amperometric Immunosensor Based on Mercapto Carboxylic Acid Self- assembled Monolayer for Phytohormone Indoleacetic Acid Assay

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments