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Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (07): 672-678. Previous Articles     Next Articles

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空壳纳米金修饰的新型DNA传感器

卜扬，杨清，孟琦，胡赢，黄杉生   

  1. (上海师范大学生命与环境科学学院 上海 200234)
  • 投稿日期:2009-06-10 修回日期:2009-09-29 发布日期:2010-04-14
  • 通讯作者: 黄杉生 E-mail:sshuang@shnu.edu.cn
  • 基金资助:

    863项目（编号2007AA06Z402），上海市科委项目（编号：0852510400），上海市重点学科资助项目（S30406），上海师范大学重点学科项目（DZL706）

A Novel DNA Biosensor Modified with Hollow Gold Nanoparticles

BO Yang, YANG Qing, MENG Qi, HU Ying, HUANG Sha-Sheng,   

  1. (Life and Environment Science College, Shanghai Normal University, Shanghai 200234)
  • Received:2009-06-10 Revised:2009-09-29 Published:2010-04-14

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A novel DNA biosensor was fabricated by modifying ssDNA, cytochrome c, L-cysteine, novel material hollow gold nanoparticles and Chitosan on the surface of glassy carbon electrode. The hollow gold nanoparticles (HGNs) were characterized by violet spectroscopy and transmission electron microscopy. The behavior of the DNA biosensor was investigated by circle voltammetry, impedance spectrum. The morphologic differences among each layer of the DNA biosensor were also observed by atomic force microscope. Results revealed that the amount of adsorbed DNA was 1.672×10―10 mol•cm－2. It could be concluded that the modified electrode could be used to detect DNA in the event of hybridization with the indicator daunomycin by differential pulse voltammetry (DPV).

Key words: hollow gold nanoparticles, ssDNA, sensor