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Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (20): 2063-2068. Previous Articles     Next Articles

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磁性荧光双功能纳米材料的合成及其用于葡萄糖的分离检测

王显祥*,1,2，钟娇懿1，刘怡1，温安祥1，单志1，杨婉身*,1   

  1. (1四川农业大学生命科学与理学院 雅安 625014)
    (2四川大学化学学院 成都 610041)
  • 投稿日期:2010-04-06 修回日期:2010-05-12 发布日期:2010-06-12
  • 通讯作者: 王显祥 E-mail:xianxiangwang@hotmail.com
  • 基金资助:

    四川省教育厅自然科学科研重点

Preparation of Magnetic and Fluorescent Bifunctional Nanocomposites and Its Application to Separation and Detection of Glucose

Wang Xianxiang*,1,2 Zhong Jiaoyi1 Liu Yi1 Wen Anxiang1 Shan Zhi1 Yang Wanshen*,1   

  1. (1 College of Life and Science, Sichuan Agricultural University, Ya an 625014)
    (2 College of Chemistry, Sichuan University, Chengdu 610041)
  • Received:2010-04-06 Revised:2010-05-12 Published:2010-06-12
  • Contact: wang xianxiang E-mail:xianxiangwang@hotmail.com

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Mercaptoacetic acid capped CdTe quantum dots and superparamagnetic nano Fe3O4 were prepared in the aqueous phase. Next, CdTe and Fe3O4 were added into a reverse microemulsion system through a certain proportion of cyclohexane, Triton-X-100 and TEOS, formed both fluorescent and magnetic bifunctional hydrophilic composite nano-materials (MQDs) after stirring 48 h at 30 ℃. Their uniform distribution of particle sizes were about 15～20 nm. Glucose oxidase (GOx) was immobilized on the surface of MQDs with crosslinking 1 h by 5% glutaraldehyde as crosslinking agent. Compared with free GOx, the optimum pH of immobilized GOx is 5.5 and the optimum temperature was 21 ℃. Using magnetisim and fluorescence of the MQDs, the simultaneous separation and fluorescence visualization detection of glucose were achieved for the samples, based on fluorescence of QDs can be quenched by the H2O2 produced by the GOx catalysis glucose.

Key words: quantum dots, magnetisim, reverse microemulsion system, glucose, detection