Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (18): 2137-2142. Previous Articles     Next Articles

Full Papers

基于核酸脱碱基位点的铽离子荧光增强型单核苷酸多态性识别研究

崔清华,邵勇*,马坤,刘桂英,吴飞,许淑娟   

  1. (浙江师范大学物理化学研究所 金华 321004)
  • 投稿日期:2011-02-13 修回日期:2011-05-10 发布日期:2011-05-20
  • 通讯作者: 邵勇 E-mail:yshao@zjnu.cn
  • 基金资助:

    国家自然科学基金;浙江省科技厅“钱江人才”计划项目;电分析化学国家重点实验室基金

Study on Recognition of Single-nucleotide Polymorphism based on Fluorescence Enhancement of Terbium Ion upon Binding at an Abasic Site in DNA

CUI Qing-Hua, SHAO Yong, MA Kun, LIU Gui-Ying, WU Fei, XU Shu-Juan   

  1. (Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004)<
  • Received:2011-02-13 Revised:2011-05-10 Published:2011-05-20
  • Contact: SHAO Yong E-mail:yshao@zjnu.cn

Recognition of single-nucleotide polymorphism (SNP) was proposed in this work on the basis of fluorescence enhancement of an inorganic ligand, terbium ion (Tb3+), upon binding at an abasic (AP) site in DNA. The AP site was deliberately introduced in a probe strand just opposite the base of interest in a target strand. Selective binding of Tb3+ at the AP site was found by observations of its characteristic fluorescence enhancement for AP site-containing DNA by comparison with fully matched DNA without the AP site. The enhancement is very dependent on the target base and the bases flanking the AP site. When the target base is G, the maximum enhancement of Tb3+ fluorescence can be obtained especially for guanines flanking the AP site. This method was successfully applied in detection of P177R mutation of codon 177 in cancer repression gene p53, manifesting its potential applications in SNP detection with signal-on fashion.

Key words: abasic stie, single-nucleotide polymorphism, terbium ion, fluorescence enhancement, molecular recognition