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Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (18): 2143-2147. Previous Articles     Next Articles

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基于聚硫堇和层层组装碳纳米管/HRP的过氧化氢传感器研究

杨绍明*，黄爱花，查文玲，魏志鹏，郑龙珍   

  1. (华东交通大学基础学院化学化工系 南昌 330013)
  • 投稿日期:2011-02-19 修回日期:2011-04-25 发布日期:2011-05-20
  • 通讯作者: 杨绍明 E-mail:yangsm@yahoo.cn
  • 基金资助:

    级联增敏型核酸适配体生物传感器研究;生物模拟膜界面纳米电化学研究;级联信号放大HRP酶电极的构筑及其在非水介质中的应用

H2O2 Biosensor Based on Poly(thionine) and Layer-by-Layer Self-assembly of Carbon Nanotubes and HRP

YANG Shao-Ming, HUANG Ai-Hua, CHA Wen-Ling, WEI Zhi-Peng, ZHENG Long-Zhen   

  1. (Department of Chemistry and Chemical Engineering, School of Basic Sciences, East China Jiaotong University, Nanchang 330013)
  • Received:2011-02-19 Revised:2011-04-25 Published:2011-05-20

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Carbon nanotubes (CNTs)/horseradish peroxidase (HRP) multilayer reagentless H2O2 biosensor based on poly(thionine) (PTH) mediator was fabricated by the combination of electro-polymerization and layer-by-layer self-assembly method. Electrochemical impedance spectroscopy was used to monitor the layer-by-layer self-assembly process of CNTs/HRP multilayer. The electrochemical behavior of the HRP biosensor was studied by the cyclic voltammetry (CV) and chronoamperometry (I-t). The effect of the number of assembled enzyme layers, work potential, pH value and CNTs were investigated on the response of HRP electrode. The biosensor shows a good linear range of 2.0×10－6～6.29×10－3 mol/L for H2O2, R＝0.998, with a detection limit of 1.0×10－6 mol/L.

Key words: poly(thionine), electro-polymerization, layer-by-layer self-assembly, biosensor, H2O2