Inhibitory Effect of Both Nano Eu2O3 and Eu3+ on Proliferation of HepG2 Cells

doi:10.6023/A1103022

Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (03): 277-283.DOI: 10.6023/A1103022 Previous Articles Next Articles

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纳米Eu₂O3 和Eu³⁺抑制人肝癌细胞HepG2 增殖的作用

刘洁, 魏春英, 杨频

山西大学化学生物学与分子工程教育部重点实验室分子科学研究所太原 030006

投稿日期:2011-03-02 修回日期:2011-09-27 发布日期:2011-11-01
基金资助:
国家自然科学基金(Nos. 21041008, 21171108)、山西省自然科学基金(No. 2009011012-2)和山西省回国留学人员基金(2011-016)资助项目.

Inhibitory Effect of Both Nano Eu₂O3 and Eu³⁺ on Proliferation of HepG2 Cells

Liu Jie, Wei Chunying, Yang Pin

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006

Received:2011-03-02 Revised:2011-09-27 Published:2011-11-01
Contact: 魏春英, 杨频 E-mail:weichuny@sxu.edu.cn; yangpin@sxu.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 21041008, 21171108), Shanxi Provincial Fund for Natural Sciences (No. 2009011012-2) and the Research Project Supported by Shanxi Scholarship Council of China (No. 2011-016).

The biological effects of rare earth elements and rare earth nanoparticles have aroused an extensive interest during the past few years. In vitro culture conditions, we reported the impact of nano Eu₂O3 and Eu³⁺ on the HepG2 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry and laser scanning confocal microscopy. The results suggested that the nano Eu₂O3 at low concentrations does not make an obvious impact on cell growth whereas it can suspend cell division, and the percentage of cells in G2/M phase increase gradually and lead to necrosis at higher concentrations (≥600 μg·mL^-1). In addition, nano Eu₂O3 can be internalized by live HepG2 cells. Eu³⁺ at low concentrations (≤100 μmol·L^-1) can slightly inhibit the growth of HepG2 cells by arresting the ratio of G₀/G₁ phase cells and triggerring apoptosis.

Key words: nano Eu₂O₃, Eu³⁺, HepG2 cells, apoptosis, rare earth

Cite this article

Liu Jie, Wei Chunying, Yang Pin. Inhibitory Effect of Both Nano Eu₂O3 and Eu³⁺ on Proliferation of HepG2 Cells[J]. Acta Chimica Sinica, 2012, 70(03): 277-283.

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纳米Eu₂O3 和Eu³⁺抑制人肝癌细胞HepG2 增殖的作用

Inhibitory Effect of Both Nano Eu₂O3 and Eu³⁺ on Proliferation of HepG2 Cells

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纳米Eu2O3 和Eu3+抑制人肝癌细胞HepG2 增殖的作用