The Fabrication of Silver Nanoparticles Using Puerarin and the Photothermal Sterilization and Diabetic Wound Healing Behavior

doi:10.6023/A24080252

Acta Chimica Sinica ›› 2024, Vol. 82 ›› Issue (11): 1150-1161.DOI: 10.6023/A24080252 Previous Articles Next Articles

Article

葛根素纳米银合成及光热杀菌和促糖尿病感染伤口愈合作用研究

王英辉^a, 王雨辉^b, 熊佳瑶^b, 苏思琪^b, 郝梦珂^b, 魏思敏^b^,^*()

a 长安大学理学院陕西西安 710064
b 陕西中医药大学陕西中药资源产业化省部共建协同创新中心秦药特色资源研究开发国家重点实验室(培育) 陕西咸阳 712083

投稿日期:2024-08-25 发布日期:2024-10-22
基金资助:
国家自然科学基金(22103007); 国家自然科学基金(82274353); 陕西省重点研发计划(2024SF-YBXM-517); 陕西省重点研发计划(2024SF-YBXM-426); 陕西省创新能力支撑计划-青年科技新星项目(2023KJXX-063); 中央高校基本科研业务费(300102124207); 陕西中医药大学2023年度科技创新人才体系建设计划(2023-LJRC-03); 秦药特色资源研究开发重点实验室开放课题项目(SUCM-QM202207)

The Fabrication of Silver Nanoparticles Using Puerarin and the Photothermal Sterilization and Diabetic Wound Healing Behavior

Yinghui Wang^a, Yuhui Wang^b, Jiayao Xiong^b, Siqi Su^b, Mengke Hao^b, Simin Wei^b()

a College of Science, Chang’an University, Xi’an 710064, China
b State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712083, China

Received:2024-08-25 Published:2024-10-22
Contact: ^*E-mail: weismiccas@163.com
Supported by:
National Natural Science Foundation of China(22103007); National Natural Science Foundation of China(82274353); Key Research and Development Plan of Shaanxi Province(2024SF-YBXM-517); Key Research and Development Plan of Shaanxi Province(2024SF-YBXM-426); Science and Technology Youth Stars Project of Shaanxi Province(2023KJXX-063); Fundamental Research Funds for the Central Universities, CHD(300102124207); Science and Technology Innovation Talent System Construction Plan of Shaanxi University of Chinese medicine(2023-LJRC-03); Open Project of State Key Laboratory of Research and Development of Characteristic Qin Medicine Resources(SUCM-QM202207)

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Abstract

The complexity of plant extract incurs the uncertainty of capping and reducing agents in the biosynthesis of silver nanoparticles (AgNPs) leading to a poor reproducibility, which dramatically limits the application of this method. To overcome this problem, the puerarin (Pue), which is an isoflavone derived from kudzu roots and has strong biological activities, is directly used as the reducing and capping agents to prepare bioactive AgNPs (Pue@AgNPs). The influence of biosynthesis parameters like pH (Pue solution), concentration of both Pue and AgNO₃ and incubation time were assessed to obtain Pue@AgNPs with superior bactericidal activity and photothermal effect. The as-prepared AgNPs are nearly spherical mainly existing in the monodispersed form with a small amount of aggregation, covering by anions (－44.0 mV) and revealing a high degree of stability (at least 15 days). It is conceived that the aggregation of Pue@AgNPs is responsible for absorbing near-infrared light (808 nm). The X-ray diﬀraction (XRD), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM) and selected area electron diﬀraction (SAED) studies show that Pue@AgNPs has a face-centered cubic (fcc) structure. The Pue@AgNPs show potent photothermal effect and antibacterial activity against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Methicillin-resistant Staphylococcus aureus (MRSA) under 808 nm laser radiation. The minimum inhibition concentration (MIC) values for E. coli, S. aureus and MRSA are 50.0 μg•mL⁻¹. The internalization of Pue@AgNPs displays a variant trend suggesting the diﬀerent mechanism of cell death. For E. coli, cell wall and intracellular damage may be responsible for cell death. However, for S. aureus and MRSA, the cell death may be rooted in oxidative stress or intracellular penetration. The Pue@AgNPs also exhibits superior antioxidation activity in trapping 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS^•+) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and could accelerate diabetic wound contraction and healing within 21 days. The histological analysis showed that Pue@AgNPs could reduce inflammation and accelerate the formation of blood and collagen deposition.

Key words: puerarin, silver nanoparticles, photothermal effect, antibacterial agent, antioxidant, wound dressing

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Yinghui Wang, Yuhui Wang, Jiayao Xiong, Siqi Su, Mengke Hao, Simin Wei. The Fabrication of Silver Nanoparticles Using Puerarin and the Photothermal Sterilization and Diabetic Wound Healing Behavior[J]. Acta Chimica Sinica, 2024, 82(11): 1150-1161.

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Fig. & Tab. 12

Figure 1. The structure of puerarin

Figure 2. UV-vis absorption spectra of the mixture at different (A) pH, (B) AgNO3 concentration, (C) Puerarin concentration and (D) incubation time

Figure 3. The average size of AgNPs at different (A) pH, (B) Puerarin concentration, (C) AgNO3 concentration and (D) incubation time; the PDI of AgNPs at different (E) pH, (F) Puerarin concentration, (G) AgNO3 concentration and (H) incubation time

Figure 4. (A) EDX spectrum; (B) and (C) TEM images; (D) average size; (E) HRTEM image; (F) XRD spectrum; (G) Zeta potential

Figure 5. (A) Infrared thermal images; (B) Photothermal heating curves of AgNPs with different concentrations; (C) infrared thermal images; (D) photothermal heating curves of AgNPs with di?erent laser power densities

Figure 6. The antimicrobial activity of AgNPs against (A) E. coli, (B) S. aureus and (C) MRSA without 808 nm laser radiation; the antimicrobial activity of AgNPs against (D) E. coli, (E) S. aureus and (F) MRSA with 808 nm laser radiation

Figure 7. Photographs of (A) E. coli, (B) S. aureus and (C) MRSA colonies grown on LB agar plates

Figure 8. AgNPs uptake analysis in (A) E. coli, (C) S. aureus and (E) MRSA without 808 nm laser radiation; AgNPs uptake analysis in (B) E. coli, (D) S. aureus and (F) MRSA with 808 nm laser radiation

Figure 9. TEM images of (A) E. coli, (B) S. aureus and (C) MRSA without 808 nm laser radiation; TEM images of (D) E. coli, (E) S. aureus and (F) MRSA with 808 nm laser radiation

Figure 10. Free radical scavenging rate of AgNPs against (A) ABTS?+, (B) DPPH radical and (C) ?OH

Figure 11. The pictures of the wound area at di?erent time of AgNPs treatment groups

Figure 12. Images of (A) H&E; (B) Masson trichromatic staining of wounds with different concentration of AgNPs at day 21

References 50

[1]	Guo, X. R.; Yin, Y. G.; Tan, Z. Q.; Liu, J. F.; Jiang, G. B. Acta Chim. Sinica 2018, 76, 387 (in Chinese).
	(郭肖茹, 阴永光, 谭志强, 刘景富, 江桂斌, 化学学报, 2018, 76, 387.) doi: 10.6023/A18020067
[2]	Chatterjee, S.; Lou, X. Y.; Liang, F.; Yang, Y. W. Coordin. Chem. Rev. 2022, 459, 214461.
[3]	Beck, F.; Loessl, M.; Baeumner, A. J. Microchim. Acta 2023, 190, 91.
[4]	Peng, H. C.; Chen, S.; Yan, B. Acta Chim. Sinica 2024, 82, 797 (in Chinese).
	(彭红超, 陈胜, 阎斌, 化学学报, 2024, 82, 797.) doi: 10.6023/A24020062
[5]	Loza, K.; Heggen, M.; Epple, M. Adv. Funct. Mater. 2020, 30, 1909260.
[6]	Oun, A. A.; Shankar, S.; Rhim, J. W. Crit. Rev. Food Sci. 2020, 60, 435.
[7]	Nguyen, N. T. T.; Nguyen, L. M.; Nguyen, T. T. T.; Liew, R. K.; Nguyen, D. T. C.; Tran, T. V. Sci. Total Environ. 2022, 827, 154160.
[8]	Wang, C.; Hong, T. T.; Cui, P. F.; Wang, J. H.; Xia, J. Adv. Drug Deliver. Rev. 2021, 175, 113818.
[9]	Xu, L.; Wang, Y. Y.; Huang, J.; Chen, C. Y.; Wang, Z. X.; Xie, H. Theranostics 2020, 10, 8996.
[10]	Mat'atková, O.; Michailidu, J.; Miskovská, A.; Kolouchová, I.; Masák, J.; Cejková, A. Biotechnol. Adv. 2022, 58, 107905.
[11]	Nie, P. H.; Zhao, Y.; Xu, H. Y. Ecotox. Environ. Safe. 2023, 253, 114636.
[12]	Godoy-Gallardo, M.; Eckhard, U.; Delgado, L. M.; Puente, Y.; Hoyos-Nogués, M.; Gil, F. J.; Perez, R. A. Bioact. Mater. 2021, 6, 4470. doi: 10.1016/j.bioactmat.2021.04.033 pmid: 34027235
[13]	Li, J.; Zhang, W.; Ji, W. H.; Wang, J. Q.; Wang, N. X.; Wu, W. X.; Wu, Q.; Hou, X. Y.; Hu, W. B.; Li, L. J. Mater. Chem. B 2021, 9, 7909.
[14]	Chen, Y. T.; Zhuo, M. P.; Wen, X. Y.; Chen, W. B.; Zhang, K. Q.; Li, M. D. Adv. Sci. 2023, 10, 2206830.
[15]	Zhao, L. P.; Zhang, X.; Wang, X. X.; Guan, X. W.; Zhang, W. F.; Ma, J. L. J. Nanobiotechnol. 2021, 19, 335.
[16]	Xu, H.; Han, P. B.; Qin, A. J.; Tang, B. Z. Acta Chim. Sinica 2023, 81, 1420 (in Chinese).
	(徐赫, 韩鹏博, 秦安, 唐本忠, 化学学报, 2023, 81, 1420.) doi: 10.6023/A23050232
[17]	Sun, H. T.; Zhang, Q.; Li, J. C.; Peng, S. J.; Wang, X. L.; Cai, R. Nano Today 2021, 37, 101073.
[18]	Zheng, B. D.; He, Q. X.; Li, X. S.; Yoon, J.; Huang, J. D. Coordin. Chem. Rev. 2021, 426, 213548.
[19]	Maleki, A.; He, J. H.; Bochani, S.; Nosrati, V.; Shahbazi, M. A.; Guo, B. L. ACS Nano 2021, 15, 18895. doi: 10.1021/acsnano.1c08334 pmid: 34870413
[20]	Hao, S. Y.; Han, H. C.; Yang, Z. Y.; Chen, M. T.; Jiang, Y. Y.; Lu, G. X.; Dong, L.; Wen, H. L.; Li, H.; Liu, J. R.; Wu, L. L.; Wang, Z.; Wang, F. L. Nano-Micro Lett. 2022, 14, 178.
[21]	Huo, J. J.; Jia, Q. Y.; Huang, H.; Zhang, J.; Li, P.; Dong, X. C.; Huang, W. Chem. Soc. Rev. 2021, 50, 8762.
[22]	Mustapha, T.; Misni, N.; Ithnin, N. R.; Daskum, A. M.; Unyah, N. Z. Int. J. Env. Res. Pub. Heal. 2022, 19, 674.
[23]	Jadoun, S.; Arif, R.; Jangid, N. K.; Meena, R. K. Environ. Chem. Lett. 2021, 19, 355.
[24]	Mikhailova, E. O. J. Funct. Biomater. 2020, 11, 84.
[25]	Alharbi, N. S.; Alsubhi, N. S.; Felimban, A. I. J. Radiat. Res. Appl. Sci. 2022, 15, 109.
[26]	Rahuman, H. B. H.; Dhandapani, R.; Narayanan, S.; Palanivel, V.; Paramasivam, R.; Subbarayalu, R.; Thangavelu, S.; Muthupandian, S. IET Nanobiotechnol. 2022, 16, 115.
[27]	Moradi, F.; Sedaghat, S.; Moradi, O.; Salmanabadi, S. A. Inorg. Nano-Met. Chem. 2021, 51, 133.
[28]	Nayak, D.; Pradhan, S.; Ashe, S.; Rauta, P. R.; Nayak, B. J. Colloid Interf. Sci. 2015, 457, 329.
[29]	Zhang, Y. Q.; Cheng, X. F.; Zhang, Y. C.; Xue, X. H.; Fu, Y. Z. Colloid. Surface. A 2013, 423, 63.
[30]	Reddy, N. V.; Li, H. Z.; Hou, T. Y.; Bethu, M. S.; Ren, Z. Q.; Zhang, Z. J. Int. J. Nanomed. 2021, 16, 15. doi: 10.2147/IJN.S265003 pmid: 33447027
[31]	He, Y.; Du, Z. Y.; Lv, H. B.; Jia, Q. F.; Tang, Z. K.; Zheng, X.; Zhang, K.; Zhao, F. H. Int. J. Nanomed. 2013, 8, 1809.
[32]	Rakib-Uz-Zaman, S. M.; Hoque Apu, E.; Muntasir, M. N.; Mowna, S. A.; Khanom, M. G.; Jahan, S. S.; Akter, N.; R. Khan, M. A.; Shuborna, N. S.; Shams, S. M.; Khan, K. Challenges 2022, 13, 18.
[33]	Anandan, M.; Poorani, G.; Boomi, P.; Varunkumar, K.; Anand, K.; Chuturgoon, A. A.; Saravanan, M.; Prabu, H. G. Process Biochem. 2019, 80, 80. doi: 10.1016/j.procbio.2019.02.014
[34]	Wang, Y. H.; Wei, S. M.; Wang, K.; Wang, Z.; Duan, J. W.; Cui, L.; Zheng, H. Y.; Wang, Y.; Wang, S. S. RSC Adv. 2020, 10, 27173.
[35]	Wei, S.; Tang, Z.; Li, H.; Zhang, K.; Song, Z. Chin. Tradit. Herb. Drugs 2019, 50, 52 (in Chinese).
	(魏思敏, 唐志书, 李慧敏, 张可可, 宋忠兴, 中草药, 2019, 50, 52.)
[36]	Wei, S. M.; Wang, Y. H.; Tang, Z. S.; Su, R.; Hu, J. H.; Guo, H.; Li, C.; Jiang, J. T.; Song, Z. X. Chem. J. Chin. Univ. 2020, 41, 1391 (in Chinese).
	(魏思敏, 王英辉, 唐志书, 苏瑞, 胡锦航, 郭惠, 李琛, 蒋金涛, 宋忠兴, 高等学校化学学报, 2020, 41, 1391.) doi: 10.7503/cjcu20190721
[37]	Wang, Y. H.; Wei, S. M. ACS Omega 2022, 7, 1494.
[38]	Wei, S. M.; Wang, Y. H.; Tang, Z. S.; Hu, J. H.; Su, R.; Lin, J. J.; Zhou, T.; Guo, H.; Wang, N.; Xu, R. R. New J. Chem. 2020, 44, 9304.
[39]	Wei, S. M.; Wang, Y. H.; Tang, Z. S.; Xu, H. B.; Wang, Z.; Yang, T.; Zou, T. Y. RSC Adv. 2021, 11, 1411.
[40]	Wei, S.; Wang, Y.; Tang, Z.; Wang, Z.; Zhang, Z.; Su, R.; Jin, R.; Song, Z. Chin. Tradit. Herb. Drugs 2020, 51, 4169 (in Chinese).
	(魏思敏, 王英辉, 唐志书, 王哲, 张珍, 苏瑞, 靳如意, 宋忠兴, 中草药, 2020, 51, 4169.)
[41]	Wang, Y.; Zou, T.; Su, R.; Wei, S. Chin. Tradit. Herb. Drugs 2022, 53, 1964 (in Chinese).
	(王英辉, 邹太艳, 苏瑞, 魏思敏, 中草药, 2022, 53, 1964.)
[42]	Wei, S. M.; Hao, M. K.; Tang, Z. S.; Zhou, T.; Zhao, F.; Wang, Y. H. RSC Adv. 2022, 12, 36115.
[43]	Wei, D. W.; Sun, W. Y.; Qian, W. P.; Ye, Y. Z.; Ma, X. Y. Carbohyd. Res. 2009, 344, 2375.
[44]	De Matos, R. A.; Courrol, L. C. Amino Acids 2017, 49, 379.
[45]	Zeng, X. L.; Chen, B. H.; Wang, L. P.; Sun, Y. X.; Jin, Z.; Liu, X. Y.; Ouyang, L. P.; Liao, Y. Bioact. Mater. 2023, 19, 653.
[46]	Han, R. M.; Tian, Y. X.; Liu, Y.; Chen, C. H.; Ai, X. C.; Zhang, J. P.; Skibsted, L. H. J. Agric. Food Chem. 2009, 57, 3780.
[47]	Menichetti, A.; Mavridi-Printezi, A.; Mordini, D.; Montalti, M. J. Funct. Biomater. 2023, 14, 244.
[48]	Li, X. S.; Lovell, J. F.; Yoon, J.; Chen, X. Y. Nat. Rev. Clin. Oncol. 2020, 17, 657.
[49]	Kumari, M.; Shukla, S.; Pandey, S.; Giri, V. P.; Bhatia, A.; Tripathi, T.; Kakkar, P.; Nautiyal, C. S.; Mishra, A. ACS Appl. Mater. Interfaces 2017, 9, 4519.
[50]	Hao, M.; Wei, S.; Su, S.; Tang, Z.; Wang, Y. ACS Appl. Mater. Interfaces 2024, 16, 24221.

葛根素纳米银合成及光热杀菌和促糖尿病感染伤口愈合作用研究

The Fabrication of Silver Nanoparticles Using Puerarin and the Photothermal Sterilization and Diabetic Wound Healing Behavior

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