Synthesis and Characterization of pH-Sensitive Copolymer mPEG-acetal-PIB and Fabrication of Hydrogel for Wound Dressing

doi:10.6023/A15050317

Acta Chim. Sinica ›› 2015, Vol. 73 ›› Issue (10): 1038-1046.DOI: 10.6023/A15050317 Previous Articles Next Articles

Article

酸敏感型嵌段共聚物mPEG-acetal-PIB的合成、表征及用于构筑水凝胶敷料

任锴^a, 何金林^a, 张明祖^a, 吴一弦^b, 倪沛红^a

a 苏州大学材料与化学化工学部苏州市大分子设计与精密合成重点实验室江苏省先进功能高分子材料设计及应用重点实验室苏州 215123;
b 北京化工大学材料科学与工程学院化工资源有效利用国家重点实验室北京 100029

投稿日期:2015-05-09 发布日期:2015-07-07
通讯作者: 倪沛红 E-mail:phni@suda.edu.cn
基金资助:
国家自然科学基金项目(No.21374066)、苏州市科技发展计划工业应用基础研究项目(No.SYG201429)、江苏省高校优势学科建设项目(PAPD)、化工资源有效利用国家重点实验室(北京化工大学)开放课题(No.CRE-2014-C-202)项目资助.

Synthesis and Characterization of pH-Sensitive Copolymer mPEG-acetal-PIB and Fabrication of Hydrogel for Wound Dressing

Ren Kai^a, He Jinlin^a, Zhang Mingzu^a, Wu Yixian^b, Ni Peihong^a

a College of Chemistry, Chemical Engineering and Materials Science, Suzhou Key Laboratory of Macromolecular Design and Precision Synthesis, Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Soochow University, Suzhou 215123;
b Beijing University of Chemical Technology, State Key Laboratory of Chemical Resource Engineering, Beijing 100029

Received:2015-05-09 Published:2015-07-07
Supported by:
roject supported by the National Natural Science Foundation of China (No. 21374066), the Suzhou Science and Technology Program for Industrial Application Foundation (No. SYG201429), a Project Funded by the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions, and the State Key Lab of Chemical Resource Engineering (Beijing University of Chemical Technology) (No. CRE-2014-C-202).

An acetal-linked amphiphilic diblock copolymer, poly(ethylene glycol) methyl ether-acetal-polyisobutene (abbreviated as mPEG-acetal-PIB), was synthesized via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) “Click” reaction. The chemical composition and structures of the copolymers were characterized by ¹H NMR and FT-IR analysis. The molecular weights and molecular weight distributions of the polymers were measured by gel permeation chromatography (GPC). The critical aggregation concentration (CAC), particle size parameters and morphologies of micelles self-assembled from mPEG-acetal-PIB in aqueous solution were determined by fluorescence probe method, dynamic light scattering (DLS), and transmission electron microscopy (TEM), respectively. The results showed that this amphiphilic diblock copolymer mPEG-acetal-PIB could self-assemble into spherical micelles in aqueous solution, and the average particle size of micelles was about 100 nm. Since the acetal group is unstable in weak acidic medium, it is anticipated that the diblock copolymer micelles can be dissociated in acidic environment. To verify this, DLS analysis was used to monitor the size change of micelles with the increase of degradation time under the acidic conditions. The degradation of acetal group in acidic environment resulted in the aggregation of PIB, which caused the increase of particle size. Subsequently, a-cyclodextrin (a-CD) was added into the micellar system to interact with PEG chain via inclusion complexation, resulting in the formation of physical-crosslinked supramolecular hydrogels. X-ray diffraction (XRD) was carried out to analyze the inclusion complexation and rheological test was used to monitor the gelation kinetics of hydrogels. The materials chosen to fabricate wound dressing should have good biocompatibility, thus in vitro cytotoxicity tests against L929 cells by MTT assays was carried out to study the biocompatibility of the copolymer and hydrogel. The results showed that the viability of L929 cells incubated with both the copolymer and hydrogel were higher than 80%, indicating that the copolymer and hydrogel displayed low cytotoxicity. This biocompatible hydrogel provides a moist and cool environment on the wounds and can be degraded in the acidic conditions, reducing the risk of inflammation. Therefore, it will possess great potential in the application of wound dressing.

Key words: “Click&rdquo, reaction, pH-response, hydrogel, wound dressing

Cite this article

Ren Kai, He Jinlin, Zhang Mingzu, Wu Yixian, Ni Peihong. Synthesis and Characterization of pH-Sensitive Copolymer mPEG-acetal-PIB and Fabrication of Hydrogel for Wound Dressing[J]. Acta Chim. Sinica, 2015, 73(10): 1038-1046.

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酸敏感型嵌段共聚物mPEG-acetal-PIB的合成、表征及用于构筑水凝胶敷料

Synthesis and Characterization of pH-Sensitive Copolymer mPEG-acetal-PIB and Fabrication of Hydrogel for Wound Dressing

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