有机化学 ›› 2017, Vol. 37 ›› Issue (4): 996-999.DOI: 10.6023/cjoc201702027 上一篇    下一篇

研究简报

伯氏致病杆菌Xenorhabdus bovienii SN52中两个新的Rhabduscin类似物

张文波a, 石丹姝a, 张春珍a, 于志国a,b   

  1. a. 沈阳农业大学植物保护学院 沈阳 110866;
    b. 辽宁省生物农药工程技术研究中心 沈阳 100866
  • 收稿日期:2017-02-20 修回日期:2017-03-08 发布日期:2017-03-14
  • 通讯作者: 于志国 E-mail:zyu@syau.edu.cn
  • 基金资助:

    辽宁省攀登学者2012资助项目.

Two New Rhabduscin Analogues from Xenorhabdus bovienii SN52

Zhang Wenboa, Shi Danshua, Zhang Chunzhena, Yu Zhiguoa,b   

  1. a. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866;
    b. Engineering & Technological Research Center of Biopesticide for Liaoning Province, Shenyang 110866
  • Received:2017-02-20 Revised:2017-03-08 Published:2017-03-14
  • Contact: 10.6023/cjoc201702027 E-mail:zyu@syau.edu.cn
  • Supported by:

    Project supported by the Liaoning Pandeng Scholar Program in 2012.

采用硅胶柱层析、凝胶柱层析和半制备高效液相色谱技术对伯氏致病杆菌Xenorhabdus bovienii SN52的次生代谢产物进行分离纯化,得到2个新的rhabduscin类似物. 通过NMR和HR-ESI-MS等波谱技术和相关文献数据对照确定了它们的结构为4-O-(4-乙酰胺-β-L-鼠李糖苷)-苯甲醛(1)和4-O-(4-乙酰胺-β-L-鼠李糖苷)-(Z)-N-苯乙烯基甲酰胺(2). 同时对化合物12的昆虫酚氧化酶抑制活性进行了评价.

关键词: 伯氏致病杆菌, rhabduscin类似物, 结构鉴定, 酚氧化酶活性

Two new rhabduscin analogues were isolated and purified from fermentation broth of Xenorhabdus bovienii SN52 by silica gel column chromatography, sephadex LH-20 chromatography and high performance preparative liquid chromatography. On the basis of spectroscopic methods (HR-ESIMS, 1D- and 2D-NMR) and by comparison with those reported previously, their structures were identified as 4-O-(4-acetamide-β-L-rhamnoside)-benzaldehyde (1) and 4-O-(4-acetamide-β-L- rhamnoside)-(Z)-N-styrylforma mide (2). Their inhibitory activities against phenoloxidase were also tested.

Key words: Xenorhabdus bovienii, rhabduscin analogues, structural identification, phenoloxidase inhibitory activity