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有机化学
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有机化学 ›› 2009, Vol. 29 ›› Issue (06): 948-955. 上一篇    下一篇

研究论文

顶空-气相色谱-质谱联用分析桂花和叶中挥发性成分

王呈仲a,b; 苏 越*,a ; 郭寅龙*,b   

  1. (a上海中医药大学中医复杂系统研究中心 上海 201203)
    (b中国科学院上海有机化学研究所上海质谱中心 上海 200032)
  • 收稿日期:2008-12-04 修回日期:2009-02-12 发布日期:2009-06-20
  • 通讯作者: 苏 越*,a ; 郭寅龙*,b

Analysis of the Volatile Components from Flowers and Leaves of Osmanthus fragrans Lour. by Headspace-GC-MS

Wang, Chengzhong a,b; Su, Yue*,a; Guo, Yinlong*,b   

  1. (a Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional
    Chinese Medicine, Shanghai 201203)
    (b Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry,
    Chinese Academy of Sciences, Shanghai 200032)
  • Received:2008-12-04 Revised:2009-02-12 Published:2009-06-20
  • Contact: Su, Yue*,a; Guo, Yinlong*,b

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被引次数

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采用谱库检索结合准确质量测定、保留指数、串联质谱技术的多维定性分析策略鉴定化合物, 能够提高定性分析的效率和准确性. 运用顶空-气相色谱-四极质谱、顶空-气相色谱-飞行时间质谱以及顶空-气相色谱-串联质谱联用技术对桂花样品进行了分析检测, 并采用多维定性分析思路对检出的挥发性成分进行了鉴定. 结果共确认出47种挥发性成分, 其中单萜类和倍半萜类化合物为主要组分. 该定性分析策略准确可靠, 可以广泛应用于复杂样品挥发性成分的定性分析中.

关键词: 准确质量测定, 桂花, 保留指数, 串联质谱, 挥发性成分, 谱库检索

A multidimensional qualitative analysis approach, consisting of accurate mass measurement, retention index, MS/MS and mass spectrum search, has been proposed to identify compounds, which could improve efficiency and accuracy of qualitative analysis. Headspace-gas chromatography-quadrupole mass spectrometry, headspace-gas chromatography-time of flight mass spectrometry and headspace-gas chroma-tography-tandem mass spectrometry have been utilized to analyze volatile components from Osmanthus fragrans Lour., which were identified by the above multidimensional qualitative analysis approach. The re-sults showed that 47 volatile compounds were totally identified, of which monoterpene and sesquiterpene compounds were the main components. This qualitative analysis strategy is accurate and reliable, and can be widely applied to qualitative analysis of the volatile components from complex samples.

Key words: volatile compone, accurate mass measurement, Osmanthus fra-grans Lour., retention index, MS/MS, mass spectrum search