尝试4 种形成焦磷酸键的方法合成了5 种结构新颖的新型烟酰胺腺嘌呤二核苷酸(NAD)类似物. 初步考察了类似物的生物活性, 发现苹果酸酶和醇脱氢酶以类似物3b 和3d 为辅酶时, 活性只有以NAD 为辅酶时的13%～30%; 而以类似物3a, 3c 和3e 为辅酶时, 这些酶的活性均极低.
侯淑华
,
刘武军
,
赵宗保
. 新型烟酰胺腺嘌呤二核苷酸(NAD)类似物的合成及其辅酶活性[J]. 有机化学, 2012
, 32(02)
: 349
-353
.
DOI: 10.6023/cjoc1106032
Four methods were tested to synthesize nicotinamide adenine dinucleotide (NAD) analogs, and 5 NAD analogs were prepared. Assays of these NAD analogs indicated that malic enzyme and alcohol dehydrogenase had about 13%～30% enzymatic activity in the presence of analog 3b or 3d, whereas significantly lower activities were observed in the presence of analog 3a, 3c or 3e.
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