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2016 , Vol. 36 >Issue 6: 1401 - 1406

DOI: https://doi.org/10.6023/cjoc201512033

研究简报

可逆识别Cu2+的吡唑啉荧光猝灭型探针及其在PK-15细胞成像中的应用

  • 张应鹏 ,
  • 尤彩霞 ,
  • 杨云裳 ,
  • 刘小育 ,
  • 郭慧琛 ,
  • 董玉莹
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  • a 兰州理工大学石油化工学院兰州 730050;
    b 中国农业科学院兰州兽医研究所兰州 730050

收稿日期: 2015-12-23

  修回日期: 2016-01-15

  网络出版日期: 2016-02-24

基金资助

甘肃省自然科学基金(No.2014GS03265)资助项目.

收起

Synthesis of Reversible Fluorescence Quenched Probe for Cu2+ and Imaging in PK-15 cells

  • Zhang Yingpeng ,
  • You Caixia ,
  • Yang Yunshang ,
  • Liu Xiaoyu ,
  • Guo Huichen ,
  • Dong Yuying
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  • aSchool of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050;
    b Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730050

Received date: 2015-12-23

  Revised date: 2016-01-15

  Online published: 2016-02-24

Supported by

Project supported by the Natural Science Foundation of Gansu Province (No. 2014GS03265 ).

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摘要

设计合成了1-(2-羟基苯基)-4-(9-蒽基)-3-苯并噻唑基吡唑啉(P1), 通过紫外吸收和荧光光谱法研究了化合物与金属离子的识别特性, 并研究了其在PK-15活细胞中进行荧光显微成像实验. 研究结果表明荧光探针P1能快速、高选择、可逆地识别Cu2+, 细胞毒性实验和荧光免疫分析实验证明, 所制备的荧光探针具有很好的生物相容性和低毒性.

关键词： 吡唑啉; 荧光探针; 铜离子; 识别; PK-15活细胞

本文引用格式

张应鹏 , 尤彩霞 , 杨云裳 , 刘小育 , 郭慧琛 , 董玉莹 . 可逆识别Cu2+的吡唑啉荧光猝灭型探针及其在PK-15细胞成像中的应用[J]. 有机化学, 2016 , 36(6) : 1401 -1406 . DOI: 10.6023/cjoc201512033

Abstract

A new pyrazoline derivative 1-(2-hydroxyphenyl)-4-(9-anthryl)-3-benzothiazole pyrazolin (P1) was designed and synthesized. The recognition properties of the target compound P1 with metal ions had been investigated by UV absorption and fluorescence spectrophotometry. The probe exhibits several favorable features, including instantaneous, reversible recognition, high sensitivity and selectivity to copper ions. Cytotoxicity test and fluorescent immunoassay results show that compound P1 has good biocompatibility and low toxicity with its IC50 value of 376.558 μmol/L.

Key words： pyrazoline; fluorescent probe; copper ions; recognition; PK-15 living cells

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