马甲子叶中两个新化合物
收稿日期: 2016-07-29
修回日期: 2016-09-22
网络出版日期: 2016-10-11
基金资助
四川省科技支撑计划（No.15ZC1978）、四川省科研院所科技成果转化（No.14010139）资助项目.
Two New Compounds from the Leaves of Paliurus ramosissimus
Received date: 2016-07-29
Revised date: 2016-09-22
Online published: 2016-10-11
Supported by
Project supported by the National Key Technology Research and Development Program of the Science and Technology Department of Sichuan Province (No. 15ZC1978), and the Sichuan Provincial Research Institute of Science and Technology Achievements Transformation Fund (No. 14010139).
研究马甲子Paliurus ramosissimus（Lour.）Poir叶子乙醇提取物的化学成分.采用硅胶、高效液相制备色谱进行分离纯化得到6个化合物，根据理化性质及NMR、HR-ESIMS等波谱技术鉴定上述化合物的结构分别为2α-O-顺式对羟基肉桂酰基-3α-羟基-27-O-反式对羟基肉桂酰基白桦脂酸（1）、2α-O-反式对羟基肉桂酰基-3α-羟基-27-O-顺式对羟基肉桂酰基白桦脂酸（2）、messagenic acid A（3）、messagenic acid B（4）、白桦脂醛（5）、β-谷甾醇（6）.其中化合物1和2为新化合物，命名为马甲子素A和马甲子素B，属于白桦脂酸型五环三萜类化合物；化合物3~6首次从该植物中发现.采用四甲基偶氮唑蓝（MTT）法对化合物1~3进行体外抗肿瘤活性测试，实验结果表明化合物1~3具有显著的体外肿瘤细胞生长抑制活性.
卢辛未 , 谢莹 , 王京 , 谭镭 , 詹雁 , 阮佳 , 徐超群 . 马甲子叶中两个新化合物[J]. 有机化学, 2017 , 37(2) : 520 -525 . DOI: 10.6023/cjoc201607044
To study chemical constituents in the ethanol extract of Paliurus ramosissimus (Lour.) Poir, two new compounds along with four known compounds were isolated and purified by repeated gel column chromatographies and high performance preparative liquid chromatography. On the basis of spectroscopic methods (HR-ESIMS, 1D-and 2D-NMR), their structures were identified as 2α-(cis-p-hydroxy-cinnamyloxy)-3α-hydroxy-27-(trans-p-hydroxy-cinnamyloxy)betulinic acid (1), 2α-(trans-p-hydroxy-cinnamyloxy)-3α-hydroxy-27-(cis-p-hydroxy-cinnamyloxy)betulinic acid (2), messagenic acid A (3), mes-sagenic acid B (4), betulinaldehyde (5) and β-sitosterol (6). Among them, compounds 1 and 2 were new compounds named as paliurusene A and paliurusene B, which belong to triterpenoids, compounds 3~6 were isolated from this plant for the first time. The anti-tumor activity of compounds 1~3 was evaluated by methyl thiazolyl tetrazolium (MTT) assays. The result showed that compounds 1~3 presented obvious cytotoxicity in vitro against the cancer cell lines.
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