一种Hg2+荧光探针的合成及其在活细胞成像中的应用
收稿日期: 2017-08-30
修回日期: 2017-11-09
网络出版日期: 2017-12-01
基金资助
江苏省自然科学基金（No.BK20140536）资助项目.
A Hg2+-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging
Received date: 2017-08-30
Revised date: 2017-11-09
Online published: 2017-12-01
Supported by
Project supported by the Natural Science Foundation of Jiangsu Province (No. BK20140536).
设计合成了一个新的罗丹明席夫碱类荧光探针1，其结构经1H NMR、13C NMR、MS和X射线单晶衍射的验证.通过紫外-可见光谱法和荧光光谱法研究了探针1在乙醇-水（V/V=7/3，HEPES 10 mmol/L，pH=7.0）中对Hg2+的识别性能.探针1通过显著的荧光增强来识别Hg2+，并具有良好的选择性和抗干扰能力.通过Job's plot和MS证明了探针1和Hg2+形成1：1的配合物.探针1的荧光强度与Hg2+浓度（0~50 μmol/L）呈良好的线性关系，可以定量地检测该范围内的Hg2+.在MGC-803活细胞中的荧光显微成像表明，探针1可检测生物体内的Hg2+.
张敏 , 肖慧丰 , 韩志湘 , 仰榴青 , 吴向阳 . 一种Hg2+荧光探针的合成及其在活细胞成像中的应用[J]. 有机化学, 2018 , 38(4) : 926 -930 . DOI: 10.6023/cjoc201708061
A novel rhodamine B Schiff-base fluorescence probe 1 was synthesized and characterized by 1H NMR, 13C NMR, MS and single-crystal X-ray analysis. The recognition properties of the probe 1 with Hg2+ ions were investigated in ethanol-water (V/V=7/3, HEPES 10 mmol/L, pH=7.0) by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The probe 1 showed remarkable "turn-on" fluorescent responses to Hg2+ with good selectivity over the other metal ions examined. The 1:1 binding model of probe 1 with Hg2+ was established by Job's plot and MS spectra. The probe can be applied to the quantitative analysis of Hg2+ with a linear range covering from 0 to 50 μmol/L. The fluorescence imaging experiments in MGC-803 living cells demonstrated that 1 could be used in biological systems to monitor Hg2+.
Key words： rhodamine B; synthesis; fluorescent probe; Hg2+; bioimaging
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