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2018 , Vol. 38 >Issue 9: 2400 - 2411

DOI: https://doi.org/10.6023/cjoc201804046

综述与进展

赖氨酸翻译后修饰蛋白质的化学(半)合成及其应用

  • 王志鹏 ,
  • 李曼 ,
  • 李珲 ,
  • 刘志华 ,
  • 李影 ,
  • 郑基深
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  • a 中国科学技术大学生命学院 合肥 230027 中国;
    b 德州农工大学化学系 得克萨斯州 77840 美国;
    c 德州农工大学植物病理与微生物学系 得克萨斯州 77843 美国;
    d 安徽省兽药饲料监察所 合肥 230091 中国

收稿日期: 2018-04-28

  修回日期: 2018-05-19

  网络出版日期: 2018-06-07

基金资助

国家自然科学基金（No.U1732161）、安徽省杰出青年科学基金（No.1808085J04）和合肥物质科学技术中心方向项目培育基金（No.2017FXCX002）资助项目.

收起

Chemical (Semi-) Synthesis and Applications of Lysine Post-Translationally Modified Proteins

  • Wang Zhipeng ,
  • Li Man ,
  • Li Hui ,
  • Liu Zhihua ,
  • Li Ying ,
  • Zheng Ji-Shen
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  • a School of Life Sciences, University of Science and Technology of China, Hefei 230027, China;
    b Department of Chemistry, Texas A & M University, Texas 77840, USA;
    c Department of Plant Pathology and Microbiology, Texas A & M University, Texas 77843, USA;
    d Anhui Supervision of Veterinay Drug and Feed, Hefei 230091, China

Received date: 2018-04-28

  Revised date: 2018-05-19

  Online published: 2018-06-07

Supported by

Project supported by the National Natural Science Foundation of China (No. U1732161), the Science and Technological Fund of Anhui Province for Outstanding Youth (No. 1808085J04) and the Innovative Program Development Foundation of Hefei Center Physical Science and Technology (No. 2017FXCX002).

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摘要

天然蛋白质的翻译后修饰是表观遗传学的重要研究方向之一.最近几年，新型赖氨酸翻译后修饰模式的发现及其对染色体结构和基因转录的重大调控作用是表观遗传学研究重点之一，引起了生物学家的广泛关注.目前发展了一系列含有特定赖氨酸翻译后修饰蛋白质的制备新方法.系统总结了制备含有新型赖氨酸翻译后修饰蛋白质的最新化学生物学方法，包括生物正交反应策略、非天然氨基酸引入策略、"非天然氨基酸修饰法"策略等，并讨论其优缺点及应用前景.

关键词： 蛋白质化学合成; 翻译后修饰; 非天然氨基酸; 生物正交反应; 蛋白质组学

本文引用格式

王志鹏 , 李曼 , 李珲 , 刘志华 , 李影 , 郑基深 . 赖氨酸翻译后修饰蛋白质的化学(半)合成及其应用[J]. 有机化学, 2018 , 38(9) : 2400 -2411 . DOI: 10.6023/cjoc201804046

Abstract

The study of native proteins with post-translational modifications (PTMs) is one of the main fields of epigenetics. The discovery of novel PTM models and their vital regulatory role for chromatin structure and gene transcription have been one of the current research focuses drawing attention of biologists especially in recent years. However, we still lack efficient strategies for the preparation of sufficient amount of native proteins with certain PTMs. The currently existing chemical biology methods are reviewed, and their advantages and disadvantages are compared, including bioorthogonal reaction technique, non-canonical amino acid incorporation, etc. Furthermore, the draft will mainly focus on the application of bioorthogonal reactions on unnatural functional groups for the incorporation of lysine PTMs.

Key words： chemical synthesis of proteins; post-translational modifications; non-canonical amino acid; bioorthogonal reaction; proteome

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