一种黄酮荧光探针对肼的识别及细胞成像
收稿日期: 2018-08-28
修回日期: 2018-11-03
网络出版日期: 2018-11-26
基金资助
河南省科技计划项目（Nos.182102311108，182102310066）、河南省高校科技创新人才计划（No.15HASTIT002）、国家自然科学基金（Nos.21702178，U1304202）资助项目.
A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells
Received date: 2018-08-28
Revised date: 2018-11-03
Online published: 2018-11-26
Supported by
Project supported by the Science and Technology Project of Henan Province (Nos. 182102311108, 182102310066), the Program for Science and Technology Innovation Talents in Universities of Henan Province (No. 15HASTIT002), and the National Natural Science Foundation of China (Nos. 21702178, U1304202).
鞠志宇 , 舒朋华 , 谢智宇 , 蒋雨晴 , 陶伟杰 , 许志红 . 一种黄酮荧光探针对肼的识别及细胞成像[J]. 有机化学, 2019 , 39(3) : 697 -702 . DOI: 10.6023/cjoc201808035
A 3-hydroxyflavonoid esters HFBA was synthesized by the reaction of 2-hydroxyacetophenone with p-methyl benzaldehyde, and its structure was characterized by NMR and high resolution mass spectrometer. The recognition behaviors of HFBA to hydrazine were investigated and the results show that HFBA exhibits good selectivity and sensitivity to N2H4 with fast response and good anti-interference ability in DMSO/PBS buffer (pH=7.4). Furthermore, the detection limit of HFBA for hydrazine was estimated to be 0.11 μmol/L, and the probe HFBA was successfully applied for the imaging of hydrazine in living cells.
Key words： flavone; fluorescence; hydrazine; biological imaging
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