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2019 , Vol. 39 >Issue 12: 3583 - 3587

DOI: https://doi.org/10.6023/cjoc201904066

研究简报

小连翘化学成分的研究

  • 李竣 ,
  • 徐晓诗 ,
  • 滕海达 ,
  • 陈玉 ,
  • 梅之南 ,
  • 杨光忠
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  • a 中南民族大学药学院 武汉 430074;
    b 中南民族大学化学与材料科学学院 武汉 430074

收稿日期: 2019-04-26

  修回日期: 2019-06-17

  网络出版日期: 2019-07-17

基金资助

国家重大新药创制科技重大专项（No.2017Z09301060）和中央高校基本科研业务费（No.CZP18004）资助项目.

收起

Chemical Constituents from Hypericum Erectum Thunb

  • Li Jun ,
  • Xu Xiaoshi ,
  • Teng Haida ,
  • Chen Yu ,
  • Mei Zhinan ,
  • Yang Guangzhong
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  • a School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074;
    b College of Chemistry and Materials Sciences, South-Central University for Nationalities, Wuhan 430074

Received date: 2019-04-26

  Revised date: 2019-06-17

  Online published: 2019-07-17

Supported by

Project supported by the National Major New Drugs Innovation and Development (No. 2017ZX09301060) and the Special Fund for Basic Scientific Research of Central Colleges, South-Central University for Nationalities (No. CZP18004).

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摘要

采用溶剂提取法和正、反相硅胶柱色谱及高效液相等多种色谱技术对小连翘化学成分进行分离纯化，并根据理化性质和现代波谱技术对其进行结构鉴定.从其氯仿提取物分离3个结构类型分别属于吡喃酮、黄酮和脂肪醇的新化合物以及15个已知化合物.以脂多糖（LPS）诱导RAW264.7细胞建立炎症模型，采用Griess法测定NO的分泌量，其中hyperiflavone（2）、isocudraniaxan-thone B（5）和2-deprenylrheediaxanthone B（6）对NO分泌表现出一定的抑制活性，其IC50分别为12.90，16.24和9.86 μmol·L-1.

关键词： 金丝桃属; 小连翘; 呫吨酮; 抗炎活性

本文引用格式

李竣 , 徐晓诗 , 滕海达 , 陈玉 , 梅之南 , 杨光忠 . 小连翘化学成分的研究[J]. 有机化学, 2019 , 39(12) : 3583 -3587 . DOI: 10.6023/cjoc201904066

Abstract

The chemical constituents of Hypericum erectum Thunb were isolated and purified by solvent extraction method, the normal and reverse phase silica gel column chromatography and semi-preparative high performance liquid chromatography. Their structures were elucidated by their physicochemical property and extensive analysis of spectroscopic data. Three new compounds which belong to α-pyranone, flavonoid and aliphatic alcohol respectively, together with 15 known compounds, were isolated from the chloroform extract of H. erectum. Inflammatory cell model was constructed by lipopolysaccharide (LPS)-stimulated RAW264.7 cell line. NO production inhibitory effects of all tested compounds were measured by Griess method. Hyperiflavone (2), isocudraniaxan-thone B (5) and 2-deprenylrheediaxanthone B (6) exhibited moderate NO production inhibitory activities with the IC50 values of 16.24, 9.86 and 12.90 μmol·L-1, respectively.

Key words： Hypericum Linn; Hypericum erectum Thunb; xanthone; anti-inflammatory activity

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