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2019 , Vol. 39 >Issue 11: 3277 - 3282

DOI: https://doi.org/10.6023/cjoc201905038

研究简报

一种响应速率匹配的双反应探针用于荧光识别硫化氢

  • 解畅 ,
  • 马趁 ,
  • 贾旭 ,
  • 张学琪 ,
  • 魏超 ,
  • 张平竹 ,
  • 李小六
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  • 河北大学化学与环境科学学院 河北省化学生物学重点实验室 药物化学与分子诊断教育部重点实验室 河北保定 071002

收稿日期: 2019-05-16

  网络出版日期: 2019-07-17

基金资助

国家自然科学基金(21778013);河北省自然科学基金(B2018201234);河北省高等学校科学技术研究(QN2017015);保定市科学研究与发展计划(16zg031);河北大学实验室开放(sy201833);河北大学实验室开放(KYZJX18144)

收起

A Response Rate Matching Dual-Reactable Probe for Fluorescent Recognition of Hydrogen Sulfide

  • Chang Xie ,
  • Chen Ma ,
  • Xu Jia ,
  • Xueqi Zhang ,
  • Chao Wei ,
  • Pingzhu Zhang ,
  • Xiaoliu Li
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  • Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding, Hebei 071002

Received date: 2019-05-16

  Online published: 2019-07-17

Supported by

the National Natural Science Foundation of China(21778013);the Natural Science Foundation of Hebei Province(B2018201234);the Colleges and Universities Science Technology Research Project of Hebei Province(QN2017015);the Science Technology Research and Development Guidance Programme Project of Baoding City(16zg031);the Open Fund of Laboratory in Hebei University(sy201833);the Open Fund of Laboratory in Hebei University(KYZJX18144)

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摘要

以6-氟-7-氨基香豆素为荧光基团，氟代叠氮和7-硝基苯并氟咱（NBD）-哌嗪为H2S反应基团和荧光淬灭基团，合成一种双反应H2S荧光探针.探针对H2S的识别性质研究表明，探针的两个反应基团与H2S响应速率匹配，探针对H2S具有高选择性和灵敏性，其荧光增强约3600倍，检测极限为4.0×10-8 mol/L.酶活性测试表明，探针可用于胱硫醚β合成酶（CBS）酶活性检测和抑制剂筛选.细胞成像实验表明，探针可用于细胞内H2S的成像研究.

关键词： 硫化氢; 响应速率; 双反应; 荧光探针

本文引用格式

解畅 , 马趁 , 贾旭 , 张学琪 , 魏超 , 张平竹 , 李小六 . 一种响应速率匹配的双反应探针用于荧光识别硫化氢[J]. 有机化学, 2019 , 39(11) : 3277 -3282 . DOI: 10.6023/cjoc201905038

Abstract

A dual-reactable H2S fluorescent probe was designed and synthesized by employing ortho-fluoro-substituted coumarin azide and 7-nitrobenzofurazan-piperazine as the H2S reactive groups and the fluorescence quenching groups. The recognition behaviors of the probe to H2S were investigated and the results showed that the probe exhibited high selectivity and sensitivity. The fluorescence off-on enhancement was ca. 3600-fold, and the detection limit was 4.0×10-8 mol/L. The results of enzyme activity test indicated that the probe could be used for cystathionine β-synthase (CBS) activity detection and inhibitor screening. Furthermore, the probe was successfully applied for the imaging of H2S in living cells.

Key words： hydrogen sulfide; response rate; dual-reactable; fluorescent probe

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