SIOC English
有机化学
高级检索

有机化学 >

2020 , Vol. 40 >Issue 9: 2888 - 2894

DOI: https://doi.org/10.6023/cjoc202002030

研究论文

一种比率型荧光探针用于成像活细胞中的过氧化氢

  • 杨敏 ,
  • 夏丽丽 ,
  • 周小琴 ,
  • 贾程利 ,
  • 吉民 ,
  • 王鹏
展开
  • a 东南大学生物科学与医学工程学院 南京 210009;
    b 中国药科大学工学院 南京 210009

收稿日期: 2020-02-23

  修回日期: 2020-05-18

  网络出版日期: 2020-06-13

基金资助

国家自然科学基金面上（No.81671745）资助项目.

收起

A Ratiometric Fluorescent Probe for Imaging Hydrogen Peroxide in Living Cells

  • Yang Min ,
  • Xia Lili ,
  • Zhou Xiaoqin ,
  • Jia Chengli ,
  • Ji Min ,
  • Wang Peng
Expand
  • a School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210009;
    b Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009

Received date: 2020-02-23

  Revised date: 2020-05-18

  Online published: 2020-06-13

Supported by

Project supported by the General Program of National Natural Science Foundation of China (No. 81671745).

Fold

摘要

过氧化氢（H2O2）以分子信号的形式在各种生理和病理过程中都起着重要作用，因此快速、高效、灵敏地检测活细胞或生物体中的H2O2具有重要意义.本研究设计了一种新型的H2O2比率型荧光探针HDP-1，其对H2O2表现出比率荧光响应.探针HDP-1在H2O2检测方面具有选择性好、灵敏度高、细胞毒性低和反应速率快等优点，同时，该探针在检测活细胞中外源性和内源性H2O2都表现出了优异的性能.

关键词： 比率型荧光探针; 外源; 内源; 过氧化氢; 活细胞成像

本文引用格式

杨敏 , 夏丽丽 , 周小琴 , 贾程利 , 吉民 , 王鹏 . 一种比率型荧光探针用于成像活细胞中的过氧化氢[J]. 有机化学, 2020 , 40(9) : 2888 -2894 . DOI: 10.6023/cjoc202002030

Abstract

Hydrogen peroxide (H2O2) plays an important role in various physiological and pathological processes in the form of molecular signals. Thus, it is of great significance to detect hydrogen peroxide in living cells or organisms quickly, efficiently and sensitively. In this study, a novel ratiometric fluorescent probe was designed to show the ratiometric fluorescence response to hydrogen peroxide. The probe showed the advantages of good selectivity, high sensitivity, low cytotoxicity and fast reaction rate in hydrogen peroxide detection. The probe also provided excellent performance in detecting both exogenous and endogenous hydrogen peroxide in living cells.

Key words： ratiometric fluorescent probe; exogenous; endogenous; hydrogen peroxide; living cell imaging

参考文献

[1] Wen, X.; Wu, J.; Wang, F.; Liu, B.; Huang, C.; Wei, Y. Free Radic. Biol. Med. 2013, 65, 402.
[2] Lou, Z.; Li, P.; Han, K. Acc. Chem. Res. 2015, 48, 1358.
[3] Balaban, R. S.; Nemoto, S.; Finkel, T. Cell 2005, 120, 483.
[4] Houstis, N.; Rosen, E. D.; Lander, E. S. Nature 2006, 440, 944.
[5] Teixeira, J. P.; de Castro, A. A.; Soares, F. V.; da Cunha, E. F. F.; Ramalho, T. C. Molecules 2019, 24, 4410.
[6] Ohshima, H.; Tatemichi, M.; Sawa, T. Arch. Biochem. Biophys. 2003, 417, 3.
[7] Zhu, H.; Fan, J. L.; Wang, B. H.; Peng, X. J. Chem. Soc. Rev. 2015, 44, 4337.
[8] Chen, X. Q.; Wang, F.; Hyun, J. Y.; Wei, T. W.; Qiang, J.; Ren, X. T.; Shin, I.; Yoon, J. Chem. Soc. Rev. 2016, 45, 2976.
[9] Tian, Q.; Chen, S.; Chen, J.; Liu, R.; Wang, Y.; Yang, X.; Ye, Y. Chin. J. Org. Chem. 2019, 39, 2089(in Chinese). (田庆, 陈双虎, 陈景龙, 刘蕊, 汪雨诗, 杨晓朋, 叶勇, 有机化学, 2019, 39, 2089.)
[10] Chen, S.; Pang, C.; Chen, X.; Yan, Z.; Huang, S.; Li, X.; Zhong, Y.; Wang, Z. Chin. J. Org. Chem. 2019, 39, 1846(in Chinese). (陈思鸿, 庞楚明, 陈孝云, 严智浩, 黄诗敏, 李香弟, 钟雅婷, 汪朝阳, 有机化学, 2019, 39, 1846.)
[11] Huang, C.; Chen, H.; Li, F.; An, S. Chin. J. Org. Chem. 2019, 39, 2467(in Chinese). (黄池宝, 陈会, 李福琴, 安思雅, 有机化学, 2019, 39, 2467.)
[12] Wen, Y.; Huo, F.; Yin, C. Chin. Chem. Lett 2019, 30, 1834.
[13] Tang, L.; Tian, M.; Chen, H.; Yan, X.; Zhong, K.; Bian, Y. Dyes Pigm. 2018, 158, 482.
[14] Purdey, M. S.; McLennan, H. J.; Sutton-McDowall, M. L.; Drumm, D. W.; Zhang, X.; Capon, P. K.; Heng, S.; Thompson, J. G.; Abell, A. D. Sens. Actuators. B 2018, 262, 750.
[15] Shen, Y.; Zhang, X.; Zhang, Y.; Wu, Y.; Zhang, C.; Chen, Y.; Jin, J.; Li, H. Sens. Actuators B. Chem. 2018, 255, 42.
[16] Qiu, X.; Xin, C.; Qin, W.; Li, Z.; Zhang, D.; Zhang, G.; Peng, B.; Han, X.; Yu, C.; Li, L.; Huang, W. Talanta 2019, 199, 628.
[17] Zhang, J.; Shi, L.; Li, Z.; Li, D.; Tian, X.; Zhang, C. Analyst 2019, 144, 36438.
[18] Chen, Z.; Li, H.; Wei, J.; Xiao, Y.; Yu, H. Chin. J. Org. Chem. 2015, 35, 789(in Chinese). (陈忠林, 李红玲, 丰驾, 肖义, 于海波, 有机化学, 2015, 35, 789.)
[19] Gao, C.; Tian, Y.; Zhang, R.; Jing, J.; Zhang, X. Anal. Chem. 2017, 89, 12945.
[20] Hou, J.; Qian, M.; Zhao, H.; Li, Y.; Liao, Y.; Han, G.; Xu, Z.; Wang, F.; Song, Y.; Liu, Y. Anal. Chim. Acta 2018, 1024, 169.
[21] Wen, Y.; Liu, K.; Yang, H.; Li, Y.; Lan, H.; Liu, Y.; Zhang, X.; Yi, T. Anal. Chem. 2014, 86, 9970.
[22] Lee, M. H.; Kim, J. S.; Sessler, J. L. Chem. Soc. Rev. 2015, 44, 4185.
[23] Huang, Y.; Zhou, Q.; Feng, Y.; Zhang, W.; Fang, G.; Fang, M.; Chen, M.; Xu, C.; Meng, X. Chem. Commun. (Cambridge. U. K.) 2018, 54, 10495.
[24] Ning, P.; Hou, L.; Feng, Y.; Xu, G.; Bai, Y.; Yu, H.; Meng, X. Chem. Commun. 2019, 55, 1782.
[25] Ning, P.; Wang, W.; Chen, M.; Feng, Y.; Meng, X. Chin. Chem. Lett. 2017, 28, 1943.
[26] Xue, X.; Zhang, Y.; Liu, Z.; Song, M.; Xing, Y.; Xiang, Q.; Wang, Z.; Tu, Z.; Zhou, Y.; Ding, K.; Xu, Y. J. Med. Chem. 2016, 59, 1565.
[27] Li, B.; Lu, L.; Zhao, M.; Lei, Z.; Zhang, F. Angew. Chem.. Int. Ed. Engl. 2018, 57, 7483.
[28] Shi, Y.; Yuan, W.; Liu, Q.; Kong, M.; Li, Z.; Feng, W.; Hu, K.; Li, F. ACS Mater. Lett. 2019, 1, 418.
[29] Pilipchuk, N. V.; Piryatinski, Y. P.; Kachkovsky, G. O.; Slominskii, Y. L.; Kachkovsky, O. D. Dyes Pigm. 2007, 73, 353.
[30] Pilipchuk, N.; Piryatinski, Y.; Kachkovsky, G.; Slominskii, Y.; Kachkovsky, O. Dyes Pigm. 2007, 74, 47.
[31] Li, X.; Tao, R. R.; Hong, L. J.; Cheng, J.; Jiang, Q.; Lu, Y. M.; Liao, M. H.; Ye, W. F.; Lu, N. N.; Han, F.; Hu, Y. Z.; Hu, Y. H. J. Am. Chem. Soc. 2015, 137, 12296.
[32] Li, H.; Yao, Q.; Fan, J.; Du, J.; Wang, J.; Peng, X. Biosens. Bioelectron. 2017, 94, 536.
[33] Xiao, H.; Li, P.; Zhang, S.; Zhang, W.; Zhang, W.; Tang, B. Chem. Commun. (Cambridge. U. K.) 2016, 52, 12741.
Options
文章导航

/

〈 〉