一种水溶液中裸眼识别HSO3-/SO32-的长波长荧光探针及其应用
收稿日期: 2020-11-30
修回日期: 2021-01-17
网络出版日期: 2021-02-26
基金资助
国家自然科学基金(21878023); 国家自然科学基金(U1608222); 及辽宁特聘教授支持计划资助项目.
A Long-Wavelength Fluorescent Probe for Naked Eye Recognition of HSO3-/SO32- in Aqueous Solution and Its Application
Received date: 2020-11-30
Revised date: 2021-01-17
Online published: 2021-02-26
Supported by
National Natural Science Foundation of China(21878023); National Natural Science Foundation of China(U1608222); and the Program for Distinguished Professor of Liaoning Province.
合成了一种(E)-2-(3-氰基-4-(3-甲酰基-4-羟基苯乙烯基)-5,5-二甲基呋喃-2(5H)-亚烷基)丙二腈衍生物CDM, 1H NMR、13C NMR和HRMS验证了其结构. CDM在MeCN/Tris (V∶V=2∶8, pH=7.4)溶液中通过荧光信号“ON-OFF”识别HSO3-/SO32-, 颜色由粉色变为无色. 探针CDM具有良好的抗干扰能力和较快的响应速度(200 s), pH适用范围为7~14, 荧光光谱和紫外吸收光谱的检测限分别为6.07和1.59 μmol/L. 实验研究表明, CDM能检测真实水样和糖样中的HSO 3-/SO32-, 并能够对活细胞中的HSO3-/SO32-进行荧光成像.
关键词： 荧光探针; HSO3-/SO32-; 长波长; 裸眼识别; 细胞成像
王超 , 王鑫 , 钟克利 , 侯淑华 , 燕小梅 , 边延江 , 汤立军 . 一种水溶液中裸眼识别HSO3-/SO32-的长波长荧光探针及其应用[J]. 有机化学, 2021 , 41(6) : 2417 -2423 . DOI: 10.6023/cjoc202011037
3-Cyano-4-(3-formyl-4-hydroxystyryl)-5,5-dimethylfuran-2(5H)-alkylene)malononitrile derivative CDM was synthesized. The correctness of its structure was verified by 1H NMR, 13C NMR and HRMS. CDM can recognize HSO3-/SO32- with the naked eye through the fluorescent signal “ON-OFF” in MeCN/Tris ( V∶V=2∶8, pH=7.4) solution, and the color changes from pink to colorless. The probe CDM has good anti-interference ability and fast response speed (200 s), the pH applicable range is 7~14, and the detection limits of the fluorescence and UV absorption spectrum are 6.07 and 1.59 μmol/L. The experimental research shows that CDM can detect HSO3-/SO32- in real water samples and sugar samples and, can perform fluorescent image HSO3-/SO32- in living cells.
Key words： fluorescent probe; HSO3-/SO32-; long wavelength; naked eye recognition; cell imaging
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