一种检测ONOO–的新型荧光探针的构建与细胞成像研究
收稿日期: 2022-03-12
修回日期: 2022-04-21
网络出版日期: 2022-05-27
基金资助
郑州大学大学生创新创业训练计划(2021cxcy215); 郑州大学本科生拔尖计划资助项目.
Construction and Cell Imaging Study of a Novel Fluorescent Probe for ONOO– Detection
Received date: 2022-03-12
Revised date: 2022-04-21
Online published: 2022-05-27
Supported by
Innovation and Entrepreneurship Training Plan for College Students of Zhengzhou University(2021cxcy215); Top Undergraduates Program of Zhengzhou University
李嘉欣 , 贺如艳 , 段森林 , 黎锦华 , 韩校净 , 叶勇 . 一种检测ONOO–的新型荧光探针的构建与细胞成像研究[J]. 有机化学, 2022 , 42(8) : 2428 -2432 . DOI: 10.6023/cjoc202203023
As an important reactive nitrogen species, peroxynitrite (ONOO–) is closely related to the occurrence of various diseases. The development of methods to detect ONOO– is of great significance for understanding its related physiological and pathological mechanisms. A novel ONOO– fluorescent probe TPE-ONOO was successfully constructed using tetraphenylethylene as a fluorophore and a pyrylium group as the specific recognition site of ONOO–. The probe showed high selectivity, strong anti-interference ability and high sensitivity to ONOO–. Cell assays suggested that the probe had low cytotoxicity and was successfully used for imaging monitoring of exogenous and endogenous ONOO– in cells. The novel probe synthesized here provides an efficient tool for studying ONOO– physiological processes.
Key words： fluorescent probe; ONOO–; tetraphenylethylene; cell imaging
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