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2023 , Vol. 43 >Issue 7: 2561 - 2566

DOI: https://doi.org/10.6023/cjoc202301012

研究简报

金核霉素生物合成途径的体内研究和关键代谢物鉴定

  • 王飞 ,
  • 金文兵 ,
  • 侯现锋 ,
  • 唐功利 ,
  • 潘海学
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  • a国科大杭州高等研究院化学与材料科学学院 杭州 310024
    b中国科学院上海有机化学研究所 上海 200032

收稿日期: 2022-11-11

  修回日期: 2023-02-24

  网络出版日期: 2023-03-17

基金资助

国家自然科学基金(32070065)

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In vivo Studies on the Biosynthetic Pathway of Aureonuclemycin and Identification of Key Metabolites

  • Fei Wang ,
  • Wenbing Jin ,
  • Xianfeng Hou ,
  • Gongli Tang ,
  • Haixue Pan
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  • aSchool of Chemistry and Materials Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024
    bShanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032
*E-mail: ;

Received date: 2022-11-11

  Revised date: 2023-02-24

  Online published: 2023-03-17

Supported by

National Natural Science Foundation of China(32070065)

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摘要

在前期获得金核霉素生物合成基因簇的基础上, 通过同框删除方法构建了全部基因敲除突变株, 确定了在金核霉素生物合成中发挥作用的关键基因. 通过突变株和野生型菌株发酵, 得到了两个关键的金核霉素类化合物. 根据体内基因敲除实验、突变株代谢物分析、关键化合物鉴定结果, 提出了金核霉素可能的生物合成途径.

关键词： 核苷类抗生素; 生物合成; 金核霉素

本文引用格式

王飞 , 金文兵 , 侯现锋 , 唐功利 , 潘海学 . 金核霉素生物合成途径的体内研究和关键代谢物鉴定[J]. 有机化学, 2023 , 43(7) : 2561 -2566 . DOI: 10.6023/cjoc202301012

Abstract

On the basis of aureonuclemycin biosynthetic gene cluster obtained in the previous work, in-frame markerless deletion mutants of all genes were constructed, and the key genes that play a role in the biosynthesis of aureonuclemycin were identified. Two key aureonuclemycin-like compounds were successfully obtained from mutant and wild-type strains. According to the results of in vivo experiments, metabolite analysis and identification of key compounds, the possible biosynthetic pathway of aureonuclemycin was proposed.

Key words： nucleoside antibiotic; biosynthesis; aureonuclemycin

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