大Stokes位移的近红外氟硼二吡咯(BODIPY)荧光探针应用于生物体中Na2S2O4的检测
收稿日期: 2022-10-14
修回日期: 2023-03-10
网络出版日期: 2023-03-24
基金资助
甘肃省高等学校产业支撑计划(2021CYZC-17); 甘肃省自然科学基金(21JR1RA227); 国家自然科学基金(21462039)
Near-Infrared Visualization Fluoroboron Dipyrrole (BODIPY) Fluore-scent Probe with Large Stokes Shift for Detecting Na2S2O4 in vivo
Received date: 2022-10-14
Revised date: 2023-03-10
Online published: 2023-03-24
Supported by
Gansu Province Industrial Support Program for Colleges(2021CYZC-17); Natural Science Foundation of Gansu Province(21JR1RA227); National Natural Science Foundation of China(21462039)
基于氟硼二吡咯(BODIPY)的骨架结构, 设计合成了两种近红外荧光探针Azo-BDP1和Azo-BDP2, 其Stokes位移分别为217和224 nm. 作为Na2S2O4的两个可视化探针, Azo-BDP1和Azo-BDP2具有高灵敏度和高选择性. 机理研究方面, 采用质谱分析证实了识别机制. 除此之外, 探索了Azo-BDP1在生物体中Na2S2O4的荧光成像.
关键词： 氟硼二吡咯(BODIPY); 大斯托克斯位移; 可视化; 近红外
赵小龙 , 郭亮武 , 李宇晴 , 冉启元 , 吴会慧 , 张祯 , 苏瀛鹏 , 周鹏鑫 , 燕娜 . 大Stokes位移的近红外氟硼二吡咯(BODIPY)荧光探针应用于生物体中Na2S2O4的检测[J]. 有机化学, 2023 , 43(7) : 2484 -2491 . DOI: 10.6023/cjoc202210011
Based on the main skeleton of fluoroboron dipyrrole (BODIPY), two near-infrared fluorescent probes Azo-BDP1 and Azo-BDP2 were rationally designed and synthesized with large Stokes shift of 217 and 224 nm, respectively. As two visualized probes for Na2S2O4, Azo-BDP1 and Azo-BDP2 exhibited high sensitivity and selectivity. The reaction-based recognition mechanism was confirmed by mass spectral analysis, in addition, fluorescence imaging studies in zebrafish embryos and zebrafish proved that the probe Azo-BDP1 is suitable for the detection of Na2S2O4 in vivo.
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