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2023 , Vol. 43 >Issue 11: 4003 - 4007

DOI: https://doi.org/10.6023/cjoc202305031

研究简报

禾谷镰刀菌次级代谢产物研究

  • 张炳文 ,
  • 林雨琦 ,
  • 薛彦青 ,
  • 王婧 ,
  • 杨文超 ,
  • 王晓峰 ,
  • 刘文
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  • a 国科大杭州高等研究院 化学与材料科学学院 杭州 310024
    b 中国科学院上海有机化学研究所 生命有机化学国家重点实验室 上海 200032
    c 华中师范大学化学学院 农药与化学生物学教育部重点实验室 武汉 430079

收稿日期: 2023-05-25

  修回日期: 2023-06-20

  网络出版日期: 2023-06-26

基金资助

科技部重大专项(2022YFC2303100); 国家自然科学基金(22193070); 国家自然科学基金(32030002); 浙江省博士后科研择优资助项目

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Study on Secondary Metabolites from Fusarium graminearum

  • Bingwen Zhang ,
  • Yuqi Lin ,
  • Yanqing Xue ,
  • Jing Wang ,
  • Wenchao Yang ,
  • Xiaofeng Wang ,
  • Wen Liu
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  • a School of Chemistry and Materials Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024
    b State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032
    c National Key Laboratory of Green Pesticide, Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079
* E-mail: ;

Received date: 2023-05-25

  Revised date: 2023-06-20

  Online published: 2023-06-26

Supported by

National Key Research and Development Program of China(2022YFC2303100); National Natural Science Foundation of China(22193070); National Natural Science Foundation of China(32030002); Postdoctoral Science Preferential Funding of Zhejiang Province

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摘要

镰刀菌属真菌产多种次级代谢产物, 且显示不同的生物功能. 基于表观遗传学研究策略, 敲除全局负调控因子kmt6基因, 从而激活禾谷镰刀菌中常规培养条件下的沉默基因. 接着对该突变株的次级代谢产物进行研究, 从中分离鉴定了化合物1~4, 其中fusarane B (1)和fusarane C (2)为新化合物. 体外抗肿瘤活性实验表明, 化合物1和3对宫颈癌细胞Hela和胰腺癌细胞Mia PaCa2两种人肿瘤细胞的生长有轻微的抑制活性. 此外, 结合体外测活和分子对接实验, 对2个聚酮类化合物2和4的生物合成途径进行了推测.

关键词： 禾谷镰刀菌; 次级代谢产物; 抗肿瘤; 生物合成

本文引用格式

张炳文 , 林雨琦 , 薛彦青 , 王婧 , 杨文超 , 王晓峰 , 刘文 . 禾谷镰刀菌次级代谢产物研究[J]. 有机化学, 2023 , 43(11) : 4003 -4007 . DOI: 10.6023/cjoc202305031

Abstract

Fusarium species are able to produce structurally diverse secondary metabolites which exhibit different biological functions. Based on the epigenetic research strategy, the global negative regulator, kmt6 gene, which is related to the expression of the secondary metabolite gene cluster, was knocked out to activate the silent gene. A chemical investigation on the cultures extract of the mutant strain led to the isolation of compounds 1~4. Among them, fusarane B (1) and fusarane C (2) are two new compounds. Furthermore, the antitumor activities of 1~4 were assayed. Compounds 1 and 3 exhibited moderate inhibitory effect on two human cancer cells of Hela and Mia PaCa2. In addition, the biosynthetic pathways of two polyketones 2 and 4 were proposed based on in vivo examination and molecular docking experiment.

Key words： Fusarium graminearum; secondary metabolites; anti-tumor; biosynthesis

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