采用硅胶、凝胶等柱色谱及制备液相色谱从羌活中分离出两个新型的线型呋喃香豆素类化合物。通过高分辨质谱（HRESIMS）和核磁共振（NMR）等光谱等解析了新化合物的结构,并使用ECD计算进一步确认了化合物1和化合物2的绝对构型。化合物1在7.5 μM时可抑制脂多糖诱导的巨噬细胞RAW264.7中炎症因子NO的表达,表现出一定的抗炎活性潜能。
胡苏
,
赵丽莲
,
李昕宇
,
叶雨蓓
,
邓赟
,
黄莉钧
,
郭大乐
. 羌活中两个新的线型呋喃香豆素类成分及其抗炎活性[J]. 有机化学, 0
: 4
-4
.
DOI: 10.6023/cjoc202505002
Two new linear furocoumarins were isolated from Notopterygium incisum using silica gel, gel Sephadex LH-20 column chromatography, and preparative high-performance liquid chromatography. The structures of the new compounds were elucidated through analysis of spectroscopic evidence, including data obtained from high-resolution electrospray ionization mass spectrometry (HRESIMS) and nuclear magnetic resonance (NMR). The absolute configurations of compounds 1 and 2 were further confirmed using electronic circular dichroism (ECD) calculations. Compound 1 demonstrated the ability to inhibit the expression of nitric oxide (NO), a pro-inflammatory factor, in lipopolysaccharide-induced RAW264.7 macrophages at a concentration of 7.5 μM, indicating its potential for anti-inflammatory activity.
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