采用基因组挖掘技术,在链霉菌Streptomyces sp. YINM00050的基因组中发现了一个二型PKS基因簇,与苯并异色烷醌类化合物alnumycin的生物合成基因簇具有较高的相似性。采用多种培养基发酵野生型YINM00050菌株,没有获得到该类化合物。通过导入全局调控质粒pSH152-tRNA-Asp-AUC,构建了突变菌株YINM00050-tRNA。对突变株进行发酵,对苯并异色烷醌类化合物进行有针对性的分离,发现了1个新的抗肿瘤活性化合物alnumycin E。
张媛
,
郭莹
,
杨明熹
,
董葵
,
赵立兴
,
马玲
,
尹敏
. 链霉菌Streptomyces sp. YINM00050中基因簇激活发现苯并异色烷醌类新化合物[J]. 有机化学, 0
: 202506021
-202506021
.
DOI: 10.6023/cjoc202506021
Through genome mining, a type II polyketide synthase (PKS) gene cluster was identified within the genome of Streptomyces sp. YINM00050. This gene cluster exhibits a high degree of similarity to the biosynthetic gene cluster responsible for the production of the benzoisochromanequinone (BIQ) compound alnumycin. Nevertheless, no BIQs were isolated from the fermentations of the wild-type strain YINM00050 using various media. Subsequently, the global regulatory plasmid pSH152-tRNA-Asp-AUC was introduced into the wild-type strain, thereby generating the mutant strain YINM00050-tRNA. The subsequent fermentation of this mutant strain enabled the targeted isolation of BIQs. Moreover, a novel anti-tumor compound, alnumycin E, was identified.
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