从农用抗生素金核霉素产生菌金色链霉菌SP-371液体发酵产物中分离得到了一个新型倍半萜内酯类化合物neopentalenolactone D1 (1),通过核磁共振(1D/2D NMR)、高分辨质谱(HR-ESI-MS)等波谱技术确定化合物1的化学结构是一个具有5/5/6 三环骨架且含有酰胺官能团的新戊丙酯菌素类天然产物。从生物合成的角度分析,化合物1可认为是新戊丙酯菌素3生物合成过程中的一个分支产物。抗菌活性测试表明化合物1其对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌没有抑制活性。
A new sesquiterpene-lactone, designated as neopentalenolactone D1 (1), featuring a 5/5/6 tricyclic system containing a carboxamide functionality, was isolated from the fermentation cultures of Streptomyces aureus SP-371, which is known for producing agricultural-bactericide aureonuclemycin. Its chemical structure was elucidated by comprehensive analysis using high-resolution electrospray ionization mass spectrometry (HRESIMS) and nuclear magnetic resonance (NMR) spectroscopic data. Its structural information indicated it is probably the shunt product of neopentalenolactone biosynthetic pathway. In antibacterial activity evaluations, compound 1 demonstrated no inhibitory effects against the tested strains including Escherichia coli (DH5α), Staphylococcus aureus, and Bacillus subtilis under the experimental conditions.
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